天然产物研究与开发 ›› 2015, Vol. 27 ›› Issue (1): 109-113.doi: 10.16333/j.1001-6880.2015.01.023

• 开发研究 • 上一篇    下一篇

响应面法优化太白贝母粗多糖超声波提取工艺研究

陈林1,2,韩林1,周浓1,2*,李花3,詹莉娟1   

  1. 1 重庆三峡学院生命科学与工程学院;2 重庆市食品基础实验教学中心,重庆 404100;3 广西大学化学化工学院,广西 531499
  • 出版日期:2015-01-31 发布日期:2015-02-12

Optimization of Ultrasonic-assisted Extraction of Crude Polysaccharide from Fritillary taipaiensis P.Y.Li Using Response Surface Methodology

CHEN Lin1,2, HAN Lin1, ZHOU Nong1,2*, LI Hua3, ZHAN Li-juan1   

  1. 1 College of Life Science and Engineering,Chongqing Three Gorges University,Chongqing 404100,China; 2 Biotechnology and Food Basic Experimental Teaching Center (Municipal Experimental Teaching Demonstration Center),Chongqing 404100,China; 3 School of Chemistry & Chemical Engineering, Guangxi University, Guangxi 531499, China
  • Online:2015-01-31 Published:2015-02-12

摘要: 对太白贝母粗多糖提取工艺的研究,为太白贝母的深入综合利用提供依据。采用超声波提取太白贝母粗多糖,在单因素试验基础上,利用响应面法对提取工艺参数进行优化研究。建立料液比、时间、超声温度之间的数学模型,通过典型性分析得出最优工艺条件为:提取时间为16 min,提取温度75 ℃,料液比为1:15,总糖的含量为0.461%。试验表明,响应面法对太白贝母粗多糖提取条件的优化是可行的,可用于实际预测。

关键词: 太白贝母, 粗多糖, 超声波提取, 响应面分析

Abstract: The aim of this study was to optimize the extraction process of crude polysaccharide from Fritillary taipaiensis P.Y.Li (FTP).On the basis of single factor experiments with the method of ultrasonic-assisted extraction,extraction parameters was investigated using response surface methodology.A mathematical model including solid/liquid ratio,ultrasonic time and extraction temperature was created.By typicality analysis,the optimized extraction conditions were as follows:ultrasonic time of 16 min,extraction temperature of 75 ℃ and solid/liquid ratio of 1:15 (g/mL).Under the optimized conditions,the extraction yield of crude polysaccharide was determined to be 0.461%.The results showed that the optimization process using response surface methodology was feasible.It can be used in practical forecasting.

Key words: Fritillary Taipaiensis P.Y.Li, crude polysaccharide, ultrasonic-assisted extraction, response surface methodology

中图分类号: 

TS201.1