关键词: 排风藤, 内生真菌, 发酵, 抗氧化活性

Abstract: DPPH· scavenging assay and ferric reducing power assay in vitro were used to evaluate the antioxidant activities of the extracts from entophytic fungal PFT-2 in Solanum cathayanum. Firstly,the antioxidant activities of ethyl acetate extract from fermentation liquid and the methanol extract of mycelium were determined. Secondly,the antioxidant activities of petroleum ether fraction,chloroform fraction,ethyl acetate fraction, n-butanol fraction,acetone fraction,methanol fraction,water surplus parts from the total methanol extract of mycelium were tested,respectively.Simultaneously V_C was used as positive control.The experimental results showed that all fractions of the methanol extraction of mycelium from entophytic fungal PFT-2 in S.cathayanum had strong scavenging capacity against DPPH·,in which ethanol fraction,acetone fraction and n-butanol fraction were almost equivalent to V_C for DPPH· scavenging activity.The IC₅₀ value of ethanol fraction for the DPPH· assay was 0.023 mg/mL,which suggested that antioxidant activity of ethanol fraction was even stronger than that of VC.Meanwhile,the above fractions all revealed strong Fe³⁺ reducing power.

Key words: Solanum cathayanum, entophytic fungal, fermentation, antioxidant activity