天然产物研究与开发 ›› 2015, Vol. 27 ›› Issue (7): 1176-1180.doi: 10.16333/j.1001-6880.2015.07.010

• 研究简报 • 上一篇    下一篇

类芽孢杆菌BD3526抑菌活性物质的初步分离及性质测定

刘玉娟1,2,韩瑨1,吴江1,刘振民1,郭本恒1,吴正钧1*   

  1. 1 乳业生物技术国家重点实验室 光明乳业股份有限公司,上海 200436;2 上海海洋大学食品学院,上海 201306
  • 出版日期:2015-07-20 发布日期:2015-07-23

Purification and Characterization of Antimicrobial Components Produced by Paenibacillus sp.Strain BD3526

LIU Yu-juan1,2,HAN Jin1,WU Jiang1,LIU Zhen-min1,GUO Ben-heng1,WU Zheng-jun1*   

  1. 1 State Key Laboratory of Dairy Biotechnology,Bright Dairy & Foods Co.Ltd.,Shanghai 200436,China; 2 College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306,China
  • Online:2015-07-20 Published:2015-07-23

摘要: 本实验以一株类芽孢杆菌BD3526为研究对象,采用丙酮浸提菌体得到抑菌活性粗品,通过Sephadex LH-20对样品进行初步纯化,结果表明粗品对藤黄微球菌CGMCC1.1848的最低抑菌浓度(MIC)为12.5 mg/mL,对金黄色葡萄球菌CGMCC1.879和单增李斯特菌CGMCC1.9136的MIC为25 mg/mL;Sephadex LH-20纯化样品对藤黄微球CGMCC1.1848的MIC为1 mg/mL,对金黄色葡萄球菌CGMCC1.879和单增李斯特菌CGMCC1.9136的MIC为2 mg/mL。样品经链霉蛋白酶处理后,活性基本消失。经加热、酸碱、胰蛋白酶、胃蛋白酶、脂肪酶、蛋白酶K处理后,活性没有明显变化。因此,类芽孢杆菌BD3526产生的是一种细菌素类的抑菌物质,该抑菌物质在食品和医疗行业有广阔的应用前景。

关键词: 类芽孢杆菌, 抑菌活性, 分离纯化, 最低抑菌浓度

Abstract: The antibacterial activity of a novel bacterial strain,identified as Paenibacillus sp.BD3526,was assayed.The antimicrobial component was extracted from Paenibacillus sp.BD3526 cells with acetone and purified on Sephadex LH-20 column.The minimum inhibitory concentrations of the crude extracts were 12.5 mg/mL for Micrococcus luteus CGMCC1.1848, 25 mg/mL for Staphylococcus aureus CGMCC1.879 and Listeria monocytogenes CGMCC1.9136,while the minimum inhibitory concentrations of the preliminarily purified fraction were 1 mg/mL for Micrococcus luteus CGMCC1.1848,2 mg/mL for Staphylococcus aureus CGMCC1.879 and Listeria monocytogenes CGMCC1.9136,respectively. The antimicrobial activity was fairly stable over a wide range of pH values,different heat treatments,but disappeared after digestion by pronase.Therefore,the antimicrobial agent produced by Paenibacillus sp.BD3526 is bacteriocin-like,and potentially useful in food and medical industry.

Key words: Paenibacillus sp., antimicrobial activity, isolation and purification, minimum inhibitory concentration

中图分类号: 

Q939.92