天然产物研究与开发 ›› 2015, Vol. 27 ›› Issue (11): 1892-1899.doi: 10.16333/j.1001-6880.2015.11.011

• 研究简报 • 上一篇    下一篇

疣孢漆斑菌发酵液的化学成分及其农药活性的研究

康敏1,郭大乐2,胡佳1,万波2,周燕2,丁立生2,顾玉诚3,邓赟1*   

  1. 1 成都中医药大学药学院 中药材标准化教育部重点实验室,四川省中药资源系统研究与开发利用重点实验室—省部共建国家重点实验室培育基地,成都 611137;2 中国科学院成都生物研究所 山地生态恢复与生物资源利用重点实验室,成都 610041;3 Syngenta Jealott’s Hill International Research Centre,Berkshire RG42 6EY,UK
  • 出版日期:2015-11-30 发布日期:2015-12-02

Chemical Constituents in the Broth of Myrothecium verrucaria with Agrochemical Activities

KANG Min1, GUO Da-le2, HU Jia1, WAN Bo2, ZHOU Yan2, DING Li-sheng2, GU Yu-cheng, DENG Yun1*   

  1. 1 Key Laboratory of Standardization of Chinese Herbal Medicine,Ministry of Education,State Key Laboratory Breeding of Systematic Research Development and Utilization of Chinese Medical Resources,Co-founded by Sichuan Province and MOST,Pharmacy Department of Chengdu University of TCM,Chengdu 611137,China; 2 Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization,Chengdu Institute of Biology,Chinese Academy of Sciences, Chengdu 610041,China; 3 Syngenta Jealott’s Hill International Research Centre,Berkshire RG42 6EY,UK
  • Online:2015-11-30 Published:2015-12-02

摘要: 对一株羌活内生真菌—疣孢漆斑菌(Myrothecium verrucaria)的发酵液进行化学成分研究,采用乙酸乙酯萃取,硅胶柱色谱和制备型HPLC等色谱手段分离、纯化,从中分离得到14个单体化合物。通过理化性质与波谱数据分析,分别鉴定为:疣孢菌素B(1)、8-乙酰基漆斑菌素H(2)、疣孢菌素M(3)、疣孢菌素J(4)、漆斑菌素D(5)、乙酰基疣孢菌素L(6)、异漆斑菌素E(7)、漆斑菌素E(8)、漆斑菌素H(9)、漆斑菌素A(10)、乙酰基漆斑菌素E(11)、Isotrichoverrin A(12)、Isotrichoverrin B(13)、疣孢菌素A(14)。其中化合物2、6、7为首次从该种真菌中分离得到。并对14个化合物进行农药活性测试,结果表明化合物10 ppm对拟南芥有显著的生长抑制作用,32 ppm对早熟禾有生长抑制作用,1000 ppm对绿棉铃虫的致死率大于70%,具有良好的除草和杀虫活性。这为进一步开发疣孢漆斑菌作为生物除草剂和杀虫剂提供理论依据。

关键词: 疣孢漆斑菌, 化学成分, 单端孢霉烯, 除草活性, 杀虫活性

Abstract: To study the chemical constituents of entophytes (Myrothecium verrucaria) broth with agrochemical activities in Notopterygium incisum Ting ex H.T.Chang,fourteen compounds were isolated from its ethyl acetate extracts by silica gel,preparative HPLC and other column chromatography.On the basis of spectra data,they were identified as verrucarin B (1),8-acetoxyroridin H (2),verrucarin M (3),verrucarin J (4),roridin D (5),verrucarin L acetate (6),isororidin E (7),Roridin E (8),Roridin H (9),Roridin A (10),roridin E acetate (11),Isotrichoverrin A (12),Isotrichoverrin B (13),verrucarin A (14).Among them,compounds 2,6 and 7 were isolated from the strain for the first time.In addition,the pesticidal and herbcidal activities of the isolated 14 compounds were tested.The results showed that most compounds had positive activities on herbicidal assays and insecticidal assays.10 ppm of compounds had significant growth inhibition for Arabidopsis thaliana, 32 ppm of compounds had significant growth inhibition for Poa annua, the lethality rate of 1000 ppm in Heliothis virescens was more than 70%.The results of this study will provide a theoretical basis for the further developing trichothecenes as biological herbicides and insecticides.

Key words: Myrothecium verrucaria, chemical constituents, trichothecenes, herbcidal activity, pesticidal activity

中图分类号: 

R284.2