天然产物研究与开发 ›› 2016, Vol. 28 ›› Issue (9): 1377-1383.doi: 10.16333/j.1001-6880.2016.9.007

所属专题: No.8

• 研究论文 • 上一篇    下一篇

黄芩苷对LPS诱导的家兔乳腺炎的保护作用

文月玲,张于,姚学萍*,沈留红,余树民,曹随忠*   

  1. 四川农业大学动物医学院,成都 611130
  • 出版日期:2016-09-30 发布日期:2017-03-24

Protective Effect of Baicalin on LPS Induced -Inflammatory Responses in Rabbit Mammary Gland Tissue

WEN Yue-ling,ZHANG Yu,YAO Xue-ping*,SHEN Liu-hong,YU Shu-min,CAO Sui-zhong*   

  1. College of Veterinary Medicine,Sichuan Agricultural University,Chengdu 611130,China
  • Online:2016-09-30 Published:2017-03-24

摘要: 研究黄芩苷对LPS诱导的家兔乳腺炎性损伤的保护作用。分娩7~10 d的50只健康新西兰泌乳家兔随机分为LPS模型组(LPS,0.25 mg/kg·BW)、黄芩苷75 mg/kg·BW组、黄芩苷150 mg/kg·BW组、黄芩苷300 mg/kg·BW组和DEX组(0.5 mg/kg·BW)。通过乳头管灌注LPS,黄芩苷和DEX分别在灌注LPS前48、24、1 h和灌注后8、32、56 h耳缘静脉注射给药。灌注LPS前50 h、灌注后6、12、24、48 h和72 h测量家兔直肠温度,采集家兔耳缘静脉血;灌注LPS前50 h、灌注后12、24 h和72 h摘取乳腺组织。采集的样品进行下列检测:(1)制作病理组织学切片观察乳腺的病理组织学变化;(2)检测血清中LDH、ALP、MPO和NAG的活性变化;(3)ELISA检测血清中促炎细胞因子IL-1、IL-6和TNF-α的含量。结果显示,与LPS模型组比较,注射黄芩苷各组:(1)乳腺组织的炎性损伤减轻;(2)血清中LDH、ALP、MPO和NAG活性降低,其中黄芩苷300 mg/kg·BW组抑制血清中酶活性升高效果最好;(3)血清中促炎细胞因子IL-1、IL-6和TNF-α水平降低,其中在6 h时血清中TNF-α水平显著低于LPS模型组(P<0.05);72 h时,血清中IL-1水平显著低于LPS模型组(P<0.05)。结果表明,黄芩苷能够有效减轻乳腺组织的炎性损伤,300 mg/kg·BW保护作用最好。

关键词: 乳腺炎动物模型, 黄芩苷, 家兔, 脂多糖, 抗炎作用

Abstract: The aim of this study was to investigate protective effect of baicalin on LPS induced rabbit mastitis.Fifty healthy rabbits after parturition 7~10 d were divided into five groups randomly,including mastitis model control group (LPS,0.25 mg/kg·BW),baicalin groups with the dose 75,150 and 300 mg/kg·BW respectively,and dexamethasone (DEX) group with dose of 0.5 mg/kg·BW DEX.LPS was respectively infused into the fourth mammary glands of rabbit via the teat duct.In the baicalin and DEX groups,baicalin and DEX were injected through the auricular veins at 48 h,24 h,1 h before LPS infusion and after 8 h,32 h,56 h respectively.The rectal temperature was measured and the auricular veins blood were collected 50 h before,and 6,12,24,48,72 h after LPS infusion respectively.The following indexes at different time point were determined:(1) The pathological change of rabbit mammary gland tissues.(2) The activities of ALP,LDH,myeloperoxidase (MPO) and N-Acetyl-β-D-Glucosaminidase (NAG) in serum.(3) The levels of TNF-α,interleukin-1 (IL-1) and interleukin-6 (IL-6) in serum were measured by ELISA.The results showed that:(1) Baicalin could reduce the histopathological damage.(2) Baicalin could reduce the activities of ALP,LDH,MPO and NAG.Baicalin group (300 mg/kg·BW) had the best effect of decreasing in the activities of ALP,LDH,MPO and NAG.(3) Baicalin could reduce the level of TNF-α,IL-1,and IL-6 in serum.The level of TNF-α in serum was significantly lower than model control group in 6 h (P<0.05).The level of IL-1 in serum was significantly lower than model control group in 72 h (P<0.05).The results demonstrated that baicalin can reduce the histopathological damage and relief body symptom.The highest potent protective effect of baicalin on LPS induced rabbit mastitis was showed at the dose of 300 mg/kg·BW.

Key words: mastitis model of animal, baicalin, rabbit, LPS, anti-inflammatory activity

中图分类号: 

S823.5