天然产物研究与开发 ›› 2017, Vol. 29 ›› Issue (7): 1204-1209.doi: 10.16333/j.1001-6880.2017.7.022

所属专题: No.3

• 开发研究 • 上一篇    下一篇

十子代平方对T2DM大鼠骨骼肌PI3K、GLUT4蛋白表达的影响

张碧溦,田春雨,喇孝瑾,徐雪梅,焦子珊,林椠,王晨斌,李继安*   

  1. 华北理工大学,唐山 063000
  • 出版日期:2017-07-29 发布日期:2017-08-11

Effects of Shizidaiping on PI3K,P-PI3K and GLUT4 Expression in Musculi Skeleti of Type 2 Diabetic Rats

ZHANG Bi-wei,TIAN Chun-yu,LA Xiao-jin,XU Xue-mei,JIAO Zi-shan,LIN Qian,WANG Chen-bin,LI Ji-an*   

  1. North China University of Science and Technology,Tangshan 063000,China
  • Online:2017-07-29 Published:2017-08-11

摘要: 本实验主要探讨十子代平方对SD大鼠2型糖尿病模型胰岛素抵抗的改善作用及骨骼肌胰岛素信号转导蛋白PI3K、P-PI3K、GLUT4表达的影响,为该方的临床应用提供实验依据。采用链脲佐菌素(STZ)联合高脂高糖饲养诱导SD大鼠2型糖尿病胰岛素抵抗模型,应用十子代平方高、中、低剂量对模型大鼠进行灌胃干预,并设立正常对照组、模型组、二甲双胍对照组,给药8周,正常组和模型组给于生理盐水灌胃。给药前、给药4周、8周后检测空腹血糖(FBG)、葡萄糖耐量试验,给药8周后检测糖化血红蛋白(GHB)、糖化血清蛋白(gsp)、C-肽(C-P),计算胰岛素抵抗指数(HOMA-IR),检测骨骼肌PI3K、P-PI3K、GLUT4蛋白的表达水平。数据采用SPSS 20.0 软件进行统计学分析。结果显示各治疗组与同期模型组比较,给药八周FBG、PG2h、AUC,有明显降低(P<0.05或P<0.01);治疗结束各给药组与模型组比较,GHB、GSP、C-肽、HOMAIR,差异明显(P<0.05或P<0.01);骨骼肌PI3K各组间差异不明显(P>0.05),P-PI3K及GLUT4蛋白表达明显高于模型组(P<0.05)。因此,十子代平方可降低T2DM模型大鼠的血糖水平、改善胰岛素抵抗(IR),其药理机制与上调骨骼肌P-PI3K和GLUT4的蛋白表达水平有关。

关键词: 十子代平方, 糖代谢, 骨骼肌胰岛素抵抗, PI3K/GLUT4

Abstract: The aim of this study was to explore the improvement function of Shizidaiping on insulin resistance of type 2 diabetic SD rats model and the influence on the expression of musculi skeleti insulin signal transduction proteins PI3K,P-PI3K and GLUT4 as well as to provide experimental basis for the clinical application of Shizidaiping.Breeding induced SD rats with type 2 diabetes insulin resistance model was developed using streptozotocin,high fat and sugar.Practice lavage intervention on model rats with high,medium and low doses of Shizidaiping and set up normal control group,model group and metformin group.Eight weeks later,practice normal saline lavage for normal group and model group.Fasting blood glucose and glucose tolerance test were carried out before using the medicine,after 4 and 8 weeks of using medicine.After 8 weeks of using the medicine,glycosylated hemoglobin (GHB),glycosylated serum protein (gsp) and c-peptide (c-p) were examined.Insulin resistance index and protein expression level of musculi skelet were calculated.Then statistical analysis was carried out using SPSS 20.0 software.Compared the treatment groups with the model groups after 8 weeks of using the medicine,it was found that FBG,PG2h and AUC decreased significantly (P<0.05 or P<0.01).After the treatment finished,there was obvious difference between the treatment groups and the model groups on GHB,GSP,C-P and HOMA-IR (P<0.05 or P<0.01).There was no obvious difference between different groups on musculi skeleti PI3K (P>0.05).The protein expression level of P-PI3K and GLUT4 of the treatment groups was significantly higher than these of model groups (P<0.05).Therefore,Shizidaiping can reduce blood glucose of type 2 diabetic rats and improve insulin resistance (IR).Its pharmacological mechanism was possibly through improving of protein expression level of P-PI3K and GLUT4 of musculi skeleti.

Key words: Shizidaiping, glycometabolism, musculi skeleti insulin resistance, PI3K/GLUT4