天然产物研究与开发 ›› 2017, Vol. 29 ›› Issue (增刊1): 77-81.doi: 10.16333/j.1001-6880.2017.S.016

• 研究简报 • 上一篇    下一篇

高碘酸钠滴定法测定麦冬药材中总糖含量

张璐欣1,李德坤2,3,周大铮2,3,杨悦武2,3,余伯阳1*,鞠爱春2,3*   

  1. 1 中国药科大学,南京 211198;2天津市中药注射剂安全性评价企业重点实验室;3天津天士力之骄药业有限公司,天津 300402
  • 出版日期:2017-08-02 发布日期:2017-08-02

Determination of Radix Radix Total Sugar Content by Titration

ZHANG Lu-Xin1,LI De-kun2,3,ZHOU Da-zheng2,3,YANG Yu-wu2,3,YU Bo-yang1*,JU Ai-chun2,3*   

  1. 1China Pharmaceutical University,Nanjing 211198,China;2Tianjin Key Laboratory of Safety Evaluation Enterprise of TCM Injections;3Tianjin Tasly Pride Pharmaceutical Co.,Ltd.,Tianjin 300402,China
  • Online:2017-08-02 Published:2017-08-02

摘要: 本实验旨在采用高碘酸钠滴定法建立麦冬药材中总糖含量的测定方法。主要过程为将药材粉末经纯化水提取且过SPE商品柱后,采用高碘酸钠滴定法,利用麦冬中的糖类在酸性、水浴条件下与NaIO4溶液反应,使得高碘酸钠消耗的硫代硫酸钠溶液减少,从而测出麦冬药材中的总糖含量。结果表明,6个批次麦冬药材总糖含量分别为623.64、529.86、548.61、633.01、482.97、557.99 mg/g。该方法经方法学考察,该含量测定方法快速、准确,可用于麦冬药材中的总糖含量测定。

关键词: 高碘酸钠滴定法, 麦冬, 总糖含量

Abstract: The aim of this study is using sodium periodate titration method to establish a determination methods of total sugar content in Radix Ophiopogonis.Medicinal powder extracted by purified water and overSPE product column,under the condition of acid,water bath and after reaction with NaIO4 solution,so that the consumption of sodium thiosulfate solution is reduced,thus calculated total sugar content.The results show 6 batches of Radix Ophiopogonis total sugar content were 623.64,529.86,548.61,633.01,482.97,557.99 mg/g.After study methodology,the determination method is rapid,accurate and can be used to determine Radix Ophiopogonis total sugar content.

Key words: titration, Radix Ophiopogonis, total sugar content