关键词: 微波, 石榴皮, 多糖, 响应曲面法, 免疫调节

Abstract: In this paper,the microwave assisted extraction of pomegranate peel polysaccharide was optimized and itsimmunomodulatory activity was studied.Based on the single factor experiment,the optimum extraction condition was determined by response surface analysis method.The solid-liquid ratio,extraction time,and microwave power were chosen as independent variables and the yield of pomegranate peel polysaccharide was selected as response value.Under the optimum extraction condition of solid-liquid ratio of 44∶1 mL/g,extraction time of 10 min,and microwave power of 450 W,the yield of pomegranate peel polysaccharide was 10.48%.In addition,the immunomodulatory effects of macrophage RAW264.7 was preliminarily verified by MTT,neutral red essay,NO release determination,and adhesion test.

Key words: microwave, pomegranate peel, polysaccharide, response surface method, immunomodulatory