天然产物研究与开发 ›› 2018, Vol. 30 ›› Issue (增刊1): 39-44.doi: 10.16333/j.1001-6880.2018.S.007

• 研究论文 • 上一篇    下一篇

HPLC一测多评法同时测定参麦注射液中10种人参皂苷的含量

熊涛1,2，董礼2，吴建国1,2，李梅2，钱广生1*   

  1. 1四川大学华西药学院，成都 610041；2华润三九（雅安）药业有限公司，雅安 625000  
  • 出版日期:2018-07-31 发布日期:2018-07-31

Simultaneous Determination of Ten Compositions in Shenmai Injection by HPLC Multi-components with Single Marker

XIONG Tao1,2, DONG Li2, WU Jian-guo1,2, LI Mei2, QIAN Guang-sheng1*   

  1. 1West China School of Pharmacy,Sichuan University,Chengdu 610041,China; 2China Resources Sanjiu(Ya’an) Pharmaceutical Co.,Ltd.,Ya’an 625000,China
  • Online:2018-07-31 Published:2018-07-31

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摘要： 建立同时快速测定参麦注射液中10种人参皂苷含量的一测多评方法，并验证该方法的准确性。以人参皂苷Rb1为内标，分别建立其它9种人参皂苷的相对校正因子，计算产品中各人参皂苷的含量，实现一测多评。同时采用外标法测定参麦注射液中各人参皂苷的含量，并比较两种方法含量测定结果误差，验证一测多评法用于参麦注射液人参皂苷检测的可行性。各人参皂苷相对校正因子重复性良好，通过比较两种测定方法检测10批参麦注射液中人参皂苷含量，结果无显著差异。以人参皂苷Rb1为内标的一测多评法，同时测定参麦注射液中10个人参皂苷含量，检测结果误差小，可以实现参麦注射液的多指标质量控制。

关键词: 参麦注射液, 人参皂苷, 高效液相, 一测多评

Abstract: It establishedmulti-components with single marker method to simultaneous rapid determining ten kinds of ginsenosides in Shengmai Injection by HPLC,and verified the accuracy.Taking ginsenoside Rb1 as internal standard substance,it established the relative correction factors among 9 kinds of ginsenosides,and then calculated the content of ginsenosides in the product.And it determined the content of ginsenosides in Shenmai injection by external standard method.Then it proved the feasibility of the determination method with multi-components with single marker through comparing error value between the two methods.The relative correction factors of the ginsenosides were good.The results of comparing between two methods through 10 batches of Shenmai injection determination showed no significant difference.The multi-components with internal standard substance of ginsenosides Rb1 for 10 kinds of ginsenosides determination in Shenmai injection performed small error.And it could realize the multi compounds quality control of Shenmai Injection.
 

Key words: Shenmai injection, ginsenosides, high performance liquid, multi-components with single marker

中图分类号: 

R917

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