天然产物研究与开发 ›› 2018, Vol. 30 ›› Issue (增刊1): 119-123.doi: 10.16333/j.1001-6880.2018.S.021

• 研究简报 • 上一篇    下一篇

HPLC法测定三种獐牙菜属植物中四种药用成分的含量

吴楠1,2,陶燕铎1,桂兰1,2,焦利进1,2,杨小兵3,于瑞涛1*   

  1. 1中国科学院藏药研究重点实验室,青海省藏药研究重点实验室,中国科学院西北高原生物研究所,西宁 810008; 2中国科学院研究生院,北京 100049; 3东莞市金美济药业有限公司,东莞 523808
  • 出版日期:2018-07-31 发布日期:2018-07-31
  • 基金资助:

    青海省重大专项(2014-GX-A3A-01);青海省科技项目(2017-HZ-811);青海省藏药研究重点实验室创新平台发展建设专项(2017-ZJ-Y11)

Content Determination of Four Medicinal Components in Three Species of Swertia by High Performance Liquid Chromatography

WU Nan1,2, TAO Yan-duo1, GUI Lan1,2, JIAO Li-jin1,2, YANG Xiao-bing3, YU Rui-tao1*   

  1. 1Key Laboratory of Tibetan Medicine Research,Qinghai Provincial Key Laboratory of Tibetan Medicine Research,Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining 810008,China; 2Graduate School of Chinese Academy of Sciences,Beijing 100049,China; 3Jinmeiji Pharmaceutical Co.Ltd,Dongguan 523808,China
  • Online:2018-07-31 Published:2018-07-31

摘要: 建立HPLC测定抱茎獐牙菜、川西獐牙菜和四数獐牙菜中马钱苷酸、獐牙菜苦苷、龙胆苦苷及芒果苷含量的方法;比较各药用成分的含量差异,为资源开发提供依据。色谱柱为Dubhe C18 (4.6 mm×250 mm,10 μm),以甲醇-0.2%甲酸水(20∶80)为流动相,流速1.0 mL/min,柱温35 ℃,检测波长为254 nm。四种成分均达到基线分离,且在各植物中含量差异较大,范围分别为54.81~109.90、1.09~24.89、1.92~29.51、1.39~15.13 mg/g。其中,马钱苷酸以抱茎獐牙菜和四数獐牙菜含量最高,獐牙菜苦苷以抱茎獐牙菜含量最高,龙胆苦苷、芒果苷以川西獐牙菜含量最高。该测定方法快速、准确,可用于獐牙菜属植物的质量评价与控制。

关键词: 高效液相色谱法, 獐牙菜属, 药用成分, 含量测定

Abstract: This research aims to establish a high performance liquid chromatography (HPLC) method for the content determination of Loganic acid,Swertiamain,Gentiopicrin,Mangiferin in Swertia franchetiana,Swertia mussotil and Swertia tetraptera,to compare the content difference of the four medicinal components and to provide basis for the resources exploitation.Chromatographic analysis was achieved on a Dubhe C18 column (4.6 mm×250 mm,10 μm).The mobile phase was methanol-0.2% formic acid in water (20∶80) at the flow rate of 1.0 mL/min.The column temperature was 35 ℃,and the detection wavelength was 254 nm.Four compounds were base-isolated and the content varies widely among different plants,the ranges were 54.81-109.90,1.09-24.89,1.92-29.51,1.39-15.13 mg/g.It can be concluded that Swertia franchetiana and Swertia tetraptera have high amounts of Loganic acid,Swertia tetraptera is rich in Swertiamain,Swertia mussotil is rich in Gentiopicrin and Mangiferin.The method is rapid and precise.It can be used for the quality evalution and control of Swertia.

Key words: HPLC, Swertia, medicinal components, Content determination

中图分类号: 

R932 Q946-3