天然产物研究与开发 ›› 2019, Vol. 31 ›› Issue (2): 264-268.doi: 10.16333/j.1001-6880.2019.2.013

• 研究简报 • 上一篇    下一篇

大叶土蜜树化学成分及抗神经炎活性研究

冯凯1,华沛2,顾琼2,何琳3,滕希峰1*,曾令杰1*   

  1. 1广东药科大学中药学院 国家中医药管理局岭南药材生产与开发重点研究室;2中山大学药学院,广州 510006;3广东药科大学医药化工学院 广东省化妆品技术研究中心,中山 528458
  • 出版日期:2019-03-07 发布日期:2019-03-07
  • 基金资助:

    国家科技重大专项(2018ZX09735010);广州市科技计划(201604020109)

Chemical constituents of Bridelia retusa and their anti-neuroinflammatory activity

FENG Kai1,HUA Pei2,GU Qiong2,HE Lin3,TENG Xi-feng1*,ZENG Ling-jie1*   

  1. 1School of Chinese Medicine,Guangdong Pharmaceutical University,State Administration of Traditional Chinese Medicine Key Laboratory for Production and Development of lingnan Medicinal Materials; 2School of Pharmaceutical Sciences,Sun Yat-Sen University,Guangzhuou 510006,China; 3School of Chemistry and Chemical Engineering,Guangdong Pharmaceutical University,Guangdong Provincial Cosmetics Engineering and Technology Research Center,Zhongshan 528458,China
  • Online:2019-03-07 Published:2019-03-07

摘要: 为研究大叶土蜜树(Bridelia retusa)茎的化学成分及其抗神经炎活性,采用色谱技术从大叶土蜜树茎部分95%乙醇提取物中分离得到11个化合物。通过核磁共振波谱、质谱以及与文献数据比较,化合物结构鉴定为没食子酸(1)、木栓酮(2)、阿魏酸二十七烷脂(3)、芥子醛(4)、丁香醛(5)、丁香脂素(6)、补骨脂素(7)、补骨脂酚(8)、二十五烷酸(9)、亚油酸(10)和1-Linoleoylglycerol(11)。其中化合物3~11为首次从土蜜树属中分离得到。对化合物1~11的抗神经炎活性进行评价,发现化合物4、5、10和11对LPS诱导BV-2细胞NO生成具有显著抑制作用,其IC50分别为12.57、8.41、5.86、5.86 μM。

关键词: 大叶土蜜树, 木栓酮, 酚类, 有机酸, 抗炎

Abstract: To investigate the chemical constituents and anti-neuroinflammatory activity from the bark of Bridelia retusa,we isolated and identified eleven compounds from the bark of B.retusa through various column chromatography.Based on the NMR and MS spectra,their structures were identified as gallic acid (1),friedelin (2),ferulic acid heptacosyl ester (3),sinapaldehyde (4),syringaldehyde (5),syringaresinol (6),psoralen (7),bakuchiol (8),pentacosanoic acid (9),telfairic acid (10),1-Linoleoylglycerol (11).Compounds 3-11 were firstly isolated from the genus Bridelia.All the isolated compounds 1-11 were evaluated for their inhibition of NO production in LPS-induced BV-2 cells.Among of them,compounds 4,5,10 and 11 exhibited potent inhibition with IC50 values of 12.57,8.41,5.86 and 5.86 μM,respectively.

Key words: Bridelia retusa, friedelin, phenols, organic acid, anti-inflammatory

中图分类号: 

R284