天然产物研究与开发 ›› 2019, Vol. 31 ›› Issue (3): 373-379.doi: 10.16333/j.1001-6880.2019.3.001

• 研究论文 •    下一篇

基于高效液相指纹图谱和化学计量法的防风药材及其伪品的鉴别

张丹1,木盼盼1,郭梅1,郑开颜1,李国川2,侯芳洁1,郭龙1*,郑玉光1*   

  1. 1河北中医学院药学院;2河北中医学院实验中心,石家庄 050200
  • 出版日期:2019-04-02 发布日期:2019-04-02
  • 基金资助:

    河北省自然科学基金青年项目(H2018423029);河北省高等学校科学技术研究项目(QN2016103);中央财政公共卫生专项“2017年中药资源普查项目”(Z135080000022);全国中药炮制技术传承基地项目(冀中医药[2017]37号);河北省第二期现代农业产业技术体系创新团队项目(HBCT2018060205);河北省中医药管理局科研计划(2015079)

Identification of Saposhnikoviae Radix and their counterfeits based on HPLC fingerprint and stoichiometry

ZHANG Dan1,MU Pan-pan1,GUO Mei1,ZHENG Kai-yan1,LI Guo-chuan2,HOU Fang-jie1,GUO Long1*,ZHENG Yu-guang1*   

  1. 1College of Pharmacy,Hebei University of Chinese Medicine; 2Department of Experimental,Hebei University of Chinese Medicine,Shijiazhuang,050200,China
  • Online:2019-04-02 Published:2019-04-02

摘要: 为对防风药材及其伪品进行鉴别,利用高效液相法(HPLC)对防风药材及其伪品中4个色原酮类成分(升麻素苷、5-O-甲基维斯阿米醇苷、升麻素、亥矛酚苷)的含量进行分析,并建立了其化学指纹图谱,标定了其中的9个共有峰,相似度分析结果发现各批次样品之间的相似度在0.087~0.997之间,说明防风及其伪品之间的差异性较大。在此基础上,以4个色原酮类成分作为原变量进行系统聚类分析和主成分分析,结果表明运用HPLC法可将正品防风和伪品防风进行鉴别。研究结果揭示了防风及其伪品在化学定量指标之间的差异,为防风药材质量和真伪进行客观综合评价提供新思路。

关键词: 防风, 高效液相色谱法, 化学计量法, 真伪鉴别

Abstract: In order to identify Saposhnikoviae Radix and its counterfeit,the content of four chromogen ketones in them were analyzed by high performance liquid chromatography (HPLC),and their chemical fingerprints were established.The results of similarity analysis showed that the similarity between the samples was 0.087-0.997,which indicated that the difference between Saposhnikoviae Radix and its adulterants was great.On the basis of this,the systematic cluster analysis and principal component analysis were carried out with four chromophotoketones as provariables.The results showed that the HPLC method could be used to identify the authentic and the counterfeit of Saposhnikoviae Radix.The results revealed the internal relationship between the chemical quantitative indexes of Saposhnikoviae Radix and its counterfeit,and provided a new idea for the objective comprehensive evaluation of the quality and authenticity of Saposhnikoviae medicinal materials.

Key words: Saposhnikoviae Radix, HPLC, stoichiometry, distinguish

中图分类号: 

R917 R282.5