关键词: 四数九里香, 民族民间药, 化学成分, 细胞毒活性, 核磁共振

Abstract: The compounds from Murraya tetramera Huang and their cytotoxic activities were investigated in this study.The ethanol extract from M.tetramera Huang were separated by chromatographic techniques.The structures of these compounds were identified by ¹H-NMR, ¹³C-NMR.They were n-24 alkanoic acid (1),9,13,17,21-tetramethyl-5-docosenoic acid (2),3-O-β-D-glucopyranosyl-kaempferol glycoside (3),psoralen(4),perillaldehyde (5),quercetin (6),methyl salicylate glucoside(7),(9S,10R,11E,13R,15Z)-9,10,13-trihydroxyoctadeca-11,15-dienoic acid（8）,2-isopropyl-5-methylphenol (9),β-sitosterol-3-O-β-D-glucopy-ranosyl (10),7-hydroxycoumarin (11),esculetin (12),β-sitosterol acetate (13),kaempferol (14).All of these compounds were isolated from this plant for the first time except compound (4) and (6).The cell inhibition rates of compounds against 5 tumor cells in vitro were studied.Compared with the positive control group,the results showed that the cell inhibition rates of compounds 3,4,5,6,7,8,10,11,12,13 and 14 showed good cytotoxicity to HL60,The cell inhibition rates of compounds 6,13 and 14 showed good cytotoxicity to SMMC7721,Compounds 1,2,3,6,7,9,11,12 showed good cytotoxicity to MCF7,and compounds 1-14 showed good cytotoxicity to A549 and MCF7.The cell inhibition rates of compounds 1-14,against A549 and SW480 were lower than the positive control group.

Key words: Murraya tetramera Huang, folk medicine, compound, cytotoxic activity, NMR