关键词: 白刺花, 生物碱, 高效液相色谱法, 指纹图谱

Abstract: To establish HPLC fingerprint and determine the contents of three kinds of alkaloids in Sophora davidii (Franch.) Skeels.The chromatographic analysis were carried out on a AgilentTC-C₁₈ column (250 mm × 4.6 mm,5 μm) with acetonitrilel-0.09% triethylamine (phosphoric acid adjusted to pH3.5) for the split 4∶96 as the mobile phase at a flow rate of 1.0 mL/min.The detect wavelength was set at 205 nm,and the column temperature was set at 35 ℃.The HPLC fingerprint of S.Davidii was established,and 14 common peaks were determined.The similarity of the sample was above 0.9.Under these chromatographic conditions,Under the chromatographic conditions,each component has a good resolution,three alkaloids(matrine,sophocarpine and oxymatrine) have a good linear relationship in the range of 6.34-101.5,6.47-103.5 and 8.38-100.5 μg/mL,respectively.The average content of three alkaloids in the roots was 8.204 8,1.237 1 and 66.147 6mg/g,respectively.Simultaneously,the content in the stems was 4.246 3,1.549 6,25.035 1 mg/g,and in the leaves it was 9.314 8,6.947 4,1.573 4 mg/g.The method is simple and accurate,and can be used for quality control of S.Davidii.

Key words: Sophora davidii, alkaloid, HPLC, fingerprint