天然产物研究与开发 ›› 2019, Vol. 31 ›› Issue (7): 1278-1284.doi: 10.16333/j.1001-6880.2019.7.023

• 开发研究 • 上一篇    下一篇

虫草发酵菌丝体对胰岛素抵抗大鼠氧化应激的缓解作用

孙梦雪1,韩雪1,杨玉辉1,唐雪1,2,成向荣1,2,施用晖1,2,乐国伟1,2*   

  1. 1江南大学食品学院;2食品营养与功能食品工程技术研究中心,无锡 214122
  • 出版日期:2019-07-29 发布日期:2019-07-29
  • 基金资助:

    “十二五”国家科技部支撑计划(2012BAD33B05);江苏省普通高校学术学位研究生科研创新计划(KYLX16-0821)

Cordyceps fermentation mycelium attenuates oxidative stress in rats with insulin resistance

SUN Meng-xue1,HAN Xue1,YANG Yu-hui1,TANG Xue1,2,CHENG Xiang-rong1,2,SHI Yong-hui1,2,LE Guo-wei1,2*   

  1. 1School of Food Science and Technology,Jiangnan University; 2Center for Nutrition and Functional Engineering,Wuxi 214122,China
  • Online:2019-07-29 Published:2019-07-29

摘要: 探究虫草发酵菌丝体对胰岛素抵抗(IR)大鼠氧化应激的影响。采用低中高剂量虫草发酵菌丝体(剂量分别为1.65 g/kg 饲料,3.30 g/kg 饲料和6.60 g/kg 饲料)分别干预IR大鼠。口服葡萄糖耐量实验观察不同剂量虫草发酵菌丝体的降糖效果,同时观察血脂、氧化应激水平的变化情况。RT-PCR法测肌肉、肝脏组织氧化应激相关基因Nrf2、HO-1和NQO1 mRNA相对表达量,Western blot 法测其蛋白相对表达量。结果发现CM干预能够显著改善IR大鼠空腹血糖、血脂和机体氧化应激水平,肌肉、肝脏组织Nrf2、HO-1和NQO1 mRNA相对表达量显著上升,肝脏Nrf2和NQO1 蛋白相对表达量显著上调,并且具有剂量依赖性。说明CM具有缓解IR大鼠氧化应激的作用。

关键词: 虫草发酵菌丝体, 地塞米松, 胰岛素抵抗, 氧化应激

Abstract: To investigate the effect of Cordyceps fermentation mycelium (CM) on oxidative stress in insulin resistant (IR) rats.IR rats were respectively treated with low,medium and high dose of CM (1.65 g/kg feed,3.30 g/kg feed and 6.60 g/kg feed).The oral glucose tolerance test was used to observe the hypoglycemic effect of different doses of CM,and the changes of blood lipid and oxidative stress were observed.The mRNA expressions of Nrf2,HO-1 and NQO1 in muscle and liver were assayed by RT-PCR,Western blot was used to detect the expressions of protein.It was found that CM can significantly improve fasting blood glucose,blood lipid and oxidative stress level in IR rats,and increase the mRNA expression of Nrf2,HO-1 and NQO1in muscle and liver,and increase the expression of Nrf2 and NQO1 protein in liver,which is dose dependent.The results suggested that CM can alleviate oxidative stress in IR rats.

Key words: Cordyceps fermentation mycelium, dexamethasone, insulin resistance, oxidative stress

中图分类号: 

Q517 R151.3 Q593