天然产物研究与开发 ›› 2019, Vol. 31 ›› Issue (增刊1): 54-58.doi: 10.16333/j.1001-6880.2019.S.010

• 研究论文 • 上一篇    下一篇

高效液相法测定明党参内生菌对其呋喃香豆素的生物转化

叶晓婉,马明莉,李宇,范淑恒,王星哲,朱大恒*   

  1. 郑州大学生命科学学院,郑州 450000
  • 出版日期:2019-08-06 发布日期:2019-08-06
  • 基金资助:

    河南省高等学校重点科研项目(19A180031);郑州大学大学生创新创业训练计划(2018cxcy412);郑州大学大学生创新创业训练计划(2017cxcy371);中国烟草总公司科技重点项目(110201402011)

Determination of the biotransformation of the furocoumarins by endophytes from Changium smyrnioides Wolff by high performance liquid chromatography

YE Xiao-wan, MA Ming-li, LI Yu, FAN Shu-heng, WANG Xing-zhe, ZHU Da-heng*   

  1. School of Life Sciences,Zhengzhou University,Zhengzhou,Henan Province,450000,China
  • Online:2019-08-06 Published:2019-08-06

摘要: 为了探究明党参内生菌对其三种呋喃香豆素成分的生物转化作用,本文采用平板培养法分离明党参内生菌株。通过HPLC法测定明党参原粉培养液经明党参内生菌发酵后欧前胡素、异欧前胡素、补骨脂素三种呋喃香豆素成分的含量。采用Promesil C18-BIO 色谱柱(4.6×250 mm,5 mm),流速1.0 mL/min、柱温30 ℃、流动相配比为乙腈∶水=80%∶20%、检测波长203 nm。菌株NMY1、PMG3、NMG10和NMJ10能提升三种呋喃香豆素成分含量,其中菌株NMJ10和NMY1的促进作用最佳;内生菌NMY1和NMJ10发酵明党参培养液在24 h时呋喃香豆素总量最高,菌株PMG3和NMG10在发酵48 h时三种呋喃香豆素成分总含量最高;菌株NMJ10、NMY1、NMG10对三种呋喃香豆素类成分总含量的增加比例分别为17.47%、17.26%、16.16%;菌株在整个发酵过程中对欧前胡素这一成分的含量有显著的提高作用,更有利于欧前胡素成分的转化合成。研究表明明党参内生菌NMY1、PMG3、NMG10和NMJ10对明党参三种呋喃香豆素成分的含量具有生物转化作用。

关键词: 明党参内生菌, 呋喃香豆素, 生物转化, 高效液相色谱

Abstract: To explore endophytes from Changium smyrnioides Wolff have biotransformation effects on its three furanocoumarins components,the endophytic strains from Changium smyrnioides Wolff were separated by plate culture method.After fermentation of Changium smyrnioides Wolff raw powder culture medium of endophytes from Changium smyrnioides Wolff,the content of three furanocoumarin components of imperatorin,isoimperatorin and psoralen was determined by HPLC. The samples were separated on a Promosil C18-BIO column (4.6 mm×250 mm,5 μm) with acetonitrile-water (80∶20) as the mobile phase,the flow rate was 1.0 mL/min,the temperature of column was 30 ℃,and the detection wavelength was 203 nm. Strains NMY1,PMG3,NMG10 and NMJ10 can increase the content of three furocoumarin components,among which the strains NMJ10 and NMY1 have the best promoting effect. The endophytes NMY1 and NMJ10 had the highest total amount of furocoumarins by fermenting the Changium smyrnioides Wolff culture liquid at 24 h,and the strains PMG3 and NMG10 had the highest total content of the three furocoumarin components at 48 h of fermentation. The increasing proportions of strains NMJ10,NMY1 and NMG10 on the total content of three furocoumarins were 17.47%,17.26% and 16.16%,respectively. The strains have a significant improvement on the content of imperatorin in the whole fermentation process,and it is more conducive to the conversion synthesis of imperatorin components. It is studied that the endophytes NMY1,PMG3,NMG10 and NMJ10 from Changium smyrnioides Wolff have biotransformation effects on the content of three furanocoumarins components.

Key words: endophytes of Changium smyrnioides Wolff, furanocoumarin, biotransformation, HPLC

中图分类号: 

Q939.96