天然产物研究与开发 ›› 2020, Vol. 32 ›› Issue (1): 47-56.doi: 10.16333/j.1001-6880.2020.1.007

• 研究论文 • 上一篇    下一篇

柚皮挥发油化学成分及对灰葡萄孢菌的抑菌活性

叶肖辰皓1,姜珊珊2,焉炳飞1*,叶敏2*   

  1. 1烟台大学化学化工学院,烟台 264005;2云南农业大学植物保护学院,昆明 650201
  • 出版日期:2020-01-28 发布日期:2020-03-25
  • 基金资助:

    国家自然科学基金(31460487)

Chemical composition and antifungal activity of essential oil from Citrus maxima against Botrytis cinerea

YEXIAO Chen-hao1,JIANG Shan-shan2,YAN Bin-fei1*,YE Min2*   

  1. 1College of Chemistry and Chemical Engineering,Yantai University,Yantai 264005,China;2 School of Crop Protection,Yunnan Agricultural University,Kunming 650201,China
  • Online:2020-01-28 Published:2020-03-25

摘要: 为探讨柚皮挥发油用于果蔬采后抑菌防腐的可能性,本研究以水蒸气蒸馏法提取柚皮挥发油,利用菌丝生长速率法测定了挥发油及其主要化学成分对灰葡萄孢菌的接触和熏蒸抑菌活性。研究结果表明,用水蒸气蒸馏法提取10 h柚皮挥发油的得率为0.32% (w/w),挥发油密度为0.66 g/cm3。通过气质联用分析发现柚皮挥发油中有8种主要化学成分,其中柠檬烯含量最高,其次是月桂烯和香柏酮。挥发油及柠檬烯和月桂烯的熏蒸抑菌活性显著高于相同剂量的接触抑菌活性,且柠檬烯的活性显著高于相同剂量的挥发油和月桂烯。由于柚皮挥发油对灰葡萄孢菌有显著的抑制作用,有必要进一步研究将其用于防治灰葡萄孢菌所致果蔬采后病害的可行性。

关键词: 柚皮, 挥发油, 化学成分, 抑菌活性, 灰葡萄孢菌

Abstract: In order to explore the possibility of essential oil from Citrus maxima used to control diseases of postharvest,essential oil was extracted from Citrus maxima peel through hydro-distillation.The antifungal activity of the essential oil against Botrytis cinerea was determined by the poison food technique and the volatile activity assay.The results showed that yield of the essential oil was about 0.32% (w/w) for 10 h extraction and density of the essential oil was 0.66 g/cm3.Eight different compositions in the essential oil were identified by using GC-MS.The major components in the essential oil detected were limonene,myrcene and nootkatone,respectively.Comparing with the poison food technique the essential oil and its two main constituents,limonene and myrcene,showed better antifungal activity against B. cinerea in volatile activity.Antifungal activity of limonene was significant higher than the essential oil andmyrcenedid at same concentration.It is necessary to explore the possibility of Citrus maxima peel essential oil used in practice of postharvest disease control because of its significant antifungal activity.

Key words: Citrus maxima peel, essential oil, chemical composition, antifungal activity, Botrytis cinerea

中图分类号: 

R284.2