天然产物研究与开发 ›› 2020, Vol. 32 ›› Issue (12): 2066-2072.doi: 10.16333/j.1001-6880.2020.12.011

• 研究简报 • 上一篇    下一篇

老翘的化学成分研究

陈梦莹,李钦,韩晶晶,李晓娅,张峰*


  

  1. 河南大学药学院,开封 475004

  • 出版日期:2020-12-28 发布日期:2020-12-24
  • 基金资助:
    金银花等2种中药饮片标准化建设(ZYBZH-Y-HEN-19);国家自然科学基金青年基金(31800287);河南省教育厅科学技术研究重点项目(19B360001)

Chemical constituents of grown Fructus Forsythiae

CHEN Meng-ying,LI Qin,HAN Jing-jing,LI Xiao-ya,ZHANG Feng*#br#

#br#
  

  1. School of Pharmacy,Henan University,Kaifeng 475004,China

  • Online:2020-12-28 Published:2020-12-24

摘要:

对老翘进行化学成分和细胞毒活性的研究。利用硅胶柱色谱、SepHadex LH-20柱色谱、ODS柱色谱和半制备HPLC等多种方法进行分离纯化,通过1H NMR、13C NMR、ESI-MS等方法解析化合物的结构。共分离鉴定得到了17个化合物,分别鉴定为五福花苷酸(1)、连翘苷(2)、芦丁(3)、(+)-松脂素-4-O-β-D-吡喃葡萄糖苷(4)、(+)-表松脂素-β-D-吡喃葡萄糖苷(5)、(+)-羟基表松脂素-4′′-β-D-葡萄糖(6)、连翘脂苷 A(7)、连翘新苷(8)、木通苯乙醇苷B(9)、(7R,8S,7′R,8′S)-8-hydroxypinoresinol-4-O-β-D-glucopyranoside(10)、木通苯乙醇苷A(11)、车前草苷A(12)、连翘酯苷E(13)、落叶松脂醇-4′-O-β-D-葡萄糖苷(14)、连翘酯苷G(15)、连翘酯苷H(16)、(+)松脂素单甲基醚-β-D-葡萄糖甙(17)。其中,化合物4、6、9、10、14、17均是首次从该属植物中分离得到。通过MTT法对17个单体化合物进行体外抗肿瘤活性筛选,活性测定结果表明化合物1~17均没有抑制肿瘤细胞增殖作用。

关键词: 老翘, 化学成分, 苯乙醇苷类, 木脂素类, 抗肿瘤

Abstract:

The chemical constituents and cytotoxic activity of grown Fructus Forsythiae were studied.The compounds were isolated and purified by silica gel column chromatography,Sephadex LH-20 column chromatography,ODS column chromatography and semi preparative HPLC.The structures of the compounds were analyzed by 1H NMR,13C NMR and ESI-MS.Seventeen compounds were identified as adoxosidic acid(1),phillyrin(2),rutin(3),(+)-pinoresinol-4-O-β-D-glucoside(4),(+)-epipinoresinol-β-D-glucoside(5),(+)-rpipinoresinol-4′′-β-D-glucoside(6),forsythoside A(7),lianqiaoxinside(8),calceolariolside B(9),(7R,8S,7′R,8′S)-8-hydroxypinoresinol-4-O-β-D-glucopyranoside(10),calceolarioside A(11),plantainoside A(12),forsythoside E(13),lariciresinol 4′-O-β-D-glucoside(14),forsythoside G(15),forsythoside H(16),(+)-pinoresinolmonomethyether-β-D-glucoside(17).Compounds 4,6,9,10,14,17 were isolated from this genus for the first time.The antitumor activities of 17 compounds were screened by MTT assay.The results showed that compounds 1-17 did not inhibit the proliferation of tumor cells.

Key words: grown Fructus Forsythiae, chemical composition, phenylethanolic glycosides, lignans, antitumor

中图分类号:  R932