天然产物研究与开发 ›› 2020, Vol. 32 ›› Issue (3): 389-397.doi: 10.16333/j.1001-6880.2020.3.006

• 研究论文 • 上一篇    下一篇

不同产地连翘叶花中主要活性成分的含量分析

原江锋1,2*,胡金婉1,王大红1,2,龚明贵1,李佩艳1   

  1. 1河南科技大学食品与生物工程学院,洛阳 471023;2东部战区总医院药理科,南京 210002
  • 出版日期:2020-03-28 发布日期:2020-05-12
  • 基金资助:

    国家自然科学基金面上项目(31870093);青年科学基金(31701665);河南省自然科学基金(172102310543)

Analysis of main active components in leaves and flowers of Forsythia suspensa from different regions

YUAN Jiang-feng1,2*,HU Jin-wan1,WANG Da-hong1,2,GONG Ming-gui1,LI Pei-yan1   

  1. 1College of Food and Bioengineering,Henan University of Science and Technology,Luoyang 471023,China; 2Department of Pharmacology,Jinling Hospital,Nanjing 210002,China
  • Online:2020-03-28 Published:2020-05-12

摘要: 通过测定不同产地连翘叶花中主要活性成分的含量,分析不同产地样品的质量差异,为连翘叶花的资源开发和综合利用提供科学依据。采用液质联用(LC-MS)技术确定28批连翘叶花中芦丁、金丝桃苷、连翘酯苷A、连翘苷、齐墩果酸和熊果酸化合物,并测定这6种活性成分的含量,对结果进行主成分分析。结果表明,不同产地连翘叶花中的主要活性成分存在一定差异;主成分分析显示山西临汾、陕西西安和河南信阳连翘叶的综合评价较好,河南栾川、山东平邑和山东日照连翘花的综合评价较好。本研究建立的方法精密度、重复性和稳定性较好,可为连翘叶花的质量评价和资源产品的开发提供参考。

关键词: 连翘叶, 连翘花, LC-MS, 活性成分, 主成分分析

Abstract: The content of main active components in Forsythia suspensa leaves and flowers from different regions were determined,and the quality difference in samples was analyzed,which provided a scientific basis for the resource development and comprehensive utilization of F. suspensa leaves and flowers.The contents including rutin,hyperoside,forsythiaside A,forsythin,oleanolic acid and ursolic acid in 28 batches of F. suspensa  leaves and flowers were determined by liquid chromatography mass spectrometry (LC-MS) technology,the results were analyzed by principal component analysis (PCA).The results showed there were some differences in the main active components in F. suspensa  leaves and flowers from different regions.PCA showed the comprehensive evaluation of F. suspensa  leaves in Shanxi Linfen,Shaanxi Xi'an and Henan Xinyang were better,and the comprehensive evaluation of F. suspensa  flowers in Henan Luanchuan,Shandong Pingyi and Shandong Rizhao were better.The method of good precision,repeatability and stability showed certain reference to the comprehensive utilization and the development of resource products of F. suspensa  leaves and F. suspensa flowers.

Key words: Forsythia suspensa leaves, Forsythia suspensa flowers;liquid chromatography mass spectrometry (LC-MS);active components;principal component analysis

中图分类号: 

Q946.91