To establish a UPLC-ESI-IT-TOF-MS method for simultaneous determination of amygdalin,hydroxysafflor yellow A,liquiritin,rutin,narirutin,niringin,neohesperidin and saikosaponin A in Xuefu Zhuyu Decoction.The chromatographic separation was on Shim-Pack XR-ODS C₁₈ column (2.0 mm ×75 mm,1.6 μm) with a mobile phase consisting of water/ formic acid (0.1%)-acetonitrile and gradient elution.ESI ionization source,combined with positive and negative scanning switch simultaneously,and IT-TOF-MS was used for qualitative and quantitative analysis.The results indicated that the linear relationship,intra-day and inter-day precision,and stability of the eight components were all good,and the average recoveries of the eight components were in the range of 89.8%-108.1%.This experimental method is simple and sensitive.which gives full play to the advantages of the IT-TOF-MS detector with good stability,accurate mass,high resolution,and accurate quantification by the external standard method of the excimer ion,and can be expected to provide a scientific basis for quality control of Xuefu Zhuyu Decoction.