天然产物研究与开发 ›› 2020, Vol. 32 ›› Issue (6): 1030-1037.doi: 10.16333/j.1001-6880.2020.6.017

• 开发研究 • 上一篇    下一篇

不同干燥方法对珠兰花挥发油成分及其生物活性的影响

赵昌恒1,2,吴永祥1,万志兵1,2*,武梦雪1,王雅莉1,曹田中2,苏兴2   

  1. 1黄山学院生命与环境科学学院,黄山 245041;2黄山雾云间生态农业开发有限公司,黄山 245200
  • 出版日期:2020-06-28 发布日期:2020-07-06
  • 基金资助:
    安徽省重点研究与开发计划(201904a06020033);黄山学院校级人才启动项目(2018xkjq013);安徽省校企合作实践教育基地项目(2019sjjd54)

Effect of different drying methods on composition and biological activities of essential oils from Chloranthus spicatus flowers

ZHAO Chang-heng1,2,WU Yong-xiang1,WAN Zhi-bing1,2*,WU Meng-xue1,WANG Ya-li1,CAO Tian-zhong2,SU Xing2   

  1. 1College of Life and Environment Science,Huangshan University,Huangshan 245041,China;2Huangshan Wuyunjian Ecological Agriculture Development Co.,Ltd.,Huangshan 245200,China

  • Online:2020-06-28 Published:2020-07-06

摘要:

为了研究不同干燥方法对珠兰花中成分种类及其功能的影响,采用水蒸气蒸馏法提取珠兰花中的挥发油,通过气相色谱-质谱联用(GC-MS)对不同干燥方法获得的挥发油进行化学成分分析,同时研究其对1,1-二苯基-2-三硝基苯肼(DPPH)自由基和2,2′-联氮-双-3-乙基苯并噻唑啉-6-磺酸(ABTS)自由基除能力、抑制微生物生长为评价指标及酪氨酸酶抑制作用,研究不同方法干燥后珠兰花挥发油抗氧化和抑菌作用、抑制酪氨酸酶作用。结果表明:2种干燥处理后珠兰花的挥发油共鉴定出62种化合物,鼓风加热干燥法鉴定出58种,阴干法鉴定出56种,共有物质52种,烯类化合物含量最高。珠兰花挥发油抗氧化活性鼓风加热干燥优于阴干,均具有一定的抑菌性,酪氨酸酶抑制作用也是鼓风加热干燥方式较优,挥发油的功能性都具有浓度效应,也表明珠兰花具有美白产品研发价值。综合而言,珠兰花挥发油成分种类丰富,鼓风加热干燥处理后其挥发油萃取效果较佳。

关键词: 珠兰花挥发油, 气相色谱-质谱联用, 抗氧化活性, 抗菌活性, 酪氨酸酶, 抑制作用 ,

Abstract:

The essential oils of Chloranthus spicatus flowers from shade drying and oven dried samples were isolated by water-steam distillation and analyzed by GC-MS.Furthermore,the antioxidant activity of volatile oil was examined using various antioxidant assays,such as 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azinobis-3-ethylbenzthiazo-line-6-sulphonate acid (ABTS) free radical scavenging assays.The volatile oil was also evaluated for antimicrobial activity by paper disc diffusion method.This study also evaluated the tyrosinase inhibitory activity of the essential oils extrated from C. spicatus flowers.The results indicated that 62 major components were identified in the essential oils from 2 different drying methods,among them,essential oils from oven dried samples identified 58 components in which the relative contents of dilute hydrocarbon were the highest,and 56 components in essential oils were identified from shade drying samples,in which the relative contents of dilute hydrocarbon were also the highest.There were 52 components in both essential oils.The antioxidant activity of essential oils from 2 different drying methods was in order of oven dried >shade drying,and the essential oils had broad-spectrum antibacterial property.The essential oils from the oven dried samples had better inhibitory effect against mushroom tyrosinase,which indicated it had potential value in developing skin whitening product.In general,oven dried is the better method to treat the flowers of C. spicatus for extracting essential oils.

Key words:

">

中图分类号:  TQ9