天然产物研究与开发 ›› 2020, Vol. 32 ›› Issue (8): 1363-1369.doi: 10.16333/j.1001-6880.2020.8.012

• 研究简报 • 上一篇    下一篇

便携式近红外测定龙胆中水溶性浸出物及龙胆苦苷含量

吴晓燕,侯晓琳,宿莹,刘战,姜雨昕,孙金,翁丽丽*


  

  1. 长春中医药大学药学院中药鉴定教研室,长春 130117

  • 出版日期:2020-08-28 发布日期:2020-09-02
  • 基金资助:
    国家中医药管理局科研基金(ZYBZH-Y-JL-25);吉林省科技厅科技发展计划(20190304105YY)

Determination of water-soluble extracts and gentiopicroside in Gentianae Radix et Rhizoma by portable near-infrared spectrometers

WU Xiao-yan,HOU Xiao-lin,SU Ying,LIU Zhan,JIANG Yu-xin,SUN Jin,WENG Li-li*#br#

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  1. Department of Traditional Chinese Medicine Identification,School of Pharmary,Changchun University of TCM,Changchun 130117,China

  • Online:2020-08-28 Published:2020-09-02

摘要: 利用近红外光谱技术,分别建立快速分析龙胆药材中水溶性浸出物及龙胆苦苷含量的定量分析模型。采用高效液相色谱法测定龙胆中龙胆苦苷含量,依据《中华人民共和国药典》2015版四部通则2201项下“水溶性浸出物测定法”测定龙胆中水溶性浸出物。采集90批龙胆样品的近红外光谱图,结合偏最小二乘法,分别建立最优定量分析模型。所建立的2个模型相关系数(R2)分别为0.933 3和0.950 5,校正均方差(RMSEC)分别为0.732 7和0.252 3,预测均方差(RMSEP)分别为0.690 6和0.234 2;用验证集样品进行模型验证,平均相对偏差分别为0.06%和0.30%。结果表明,近红外光谱法可用于龙胆中水溶性浸出物及活性成分的定量分析,其处理简单、操作简便、快速无损,且结果较准确。该方法在龙胆药材的质量控制及在线监测等方面具有广阔的应用前景。


关键词: 近红外光谱法, 龙胆, 水溶性浸出物, 龙胆苦苷

Abstract:

The objective of this study was to establish a quantitative analysis model for the content of water-soluble extracts and gentiopicroside in Gentianae Radix et Rhizoma by near-infrared spectroscopy (NIR).The content of gentiopicroside in Gentianae Radix et Rhizoma was determined by high performance liquid chromatography (HPLC).The content of water-soluble extracts in Gentianae Radix et Rhizoma was determined according to the 2201 general rules of four general rules of Chinese Pharmacopoeia (2015 edition).The near-infrared spectra of 90 batches of Gentianae Radix et Rhizoma samples were collected and the optimal quantitative analysis models were established by partial least square method (PLS).The correlation coefficient (R2) in the two models were 0.933 3 and 0.950 5,respectively.The root mean square error of calibration (RMSEC) were 0.732 7 and 0.252 3,and the root mean square error of prediction (RMSEP) were 0.690 6 and 0.234 2,respectively.The average relative deviation was 0.06% and 0.30%,respectively.The results showed that the near-infrared spectroscopy can be used for quantitative analysis of water-soluble extracts and active components in Gentianae Radix et Rhizoma.The method is simple,easy to operate,fast and non-destructive,and the results are accurate.The method has a broad application prospect in quality control and online monitoring of Gentianae Radix et Rhizoma.

Key words: near-infrared spectroscopy, Gentianae Radix et Rhizoma, water-soluble extracts, gentiopicroside

中图分类号:  R282.5