天然产物研究与开发 ›› 2020, Vol. 32 ›› Issue (9): 1539-1544.doi: 10.16333/j.1001-6880.2020.9.012

• 研究简报 • 上一篇    下一篇

广东红冬蛇菰及其内生真菌Penicillium coprophilum Mzz9的化学成分研究

范升,陆盛胜,吴秋艺,李芳芳,何坚*   

  1. 南方医科大学药学院多肽与天然药物研究室,广州 510515

  • 出版日期:2020-09-28 发布日期:2020-09-25
  • 基金资助:
    国家自然科学基金(81773556);广东省科技厅项目(2015A020211010)

Studies on the chemical constituents of Balanophora harlandii and its endophytic fungus Penicillium coprophilum Mzz9

FAN Sheng,LU Sheng-sheng,WU Qiu-yi,LI Fang-fang,HE Jian*   

  1. School of Pharmaceutical Sciences,Southern Medical University,Guangzhou 510515,China

  • Online:2020-09-28 Published:2020-09-25

摘要:

本文研究了广东梅州地区产名贵中药材红冬蛇菰及其内生真菌Penicillium coprophilum Mzz9的化学成分。通过多种色谱技术和波谱学分析共分离鉴定了11个化合物。从红冬蛇菰的二氯甲烷萃取物中分离得到5个化合物:羽扇豆醇(1)、β-香树脂醇(2)、鄁桐甾醇(3)、乙酸蛇麻脂醇酯(4)和亚油酸甘油酯(5);从其内生真菌P. coprophilum Mzz9的发酵液中分离得到6个化合物:去氯灰黄霉素(6)、灰黄霉素(7)、脱氢灰黄霉素(8)、oxaline(9)、4-megastigmen-3,9-dione(10)、对羟基苯乙酮(11)。其中化合物1和5为首次从红冬蛇菰中分离得到,化合物6~11为首次从红冬蛇菰的内生真菌中分离得到。

关键词: 红冬蛇菰, 内生真菌, Penicillium coprophilum Mzz9, 次生代谢产物, 化学成分

Abstract:

This paper investigated the chemical constituents from a valuable traditional Chinese medicine,Balanophora harlandii, collected in Meizhou,Guangdong Province,as well as the secondary metabolites from the culture broth of its endophytic fungus Penicillium coprophilum Mzz9.As a result,eleven compounds were isolated and identified by combining various chromatographic and spectroscopic techniques.Among them,five compounds including lupeol (1),β-amyrin (2),clearosterol (3),lupeol acetate (4) and glycerin linoleate (5) were isolated from the dichloromethane extracts of Balanophora harlandii,while the other six compounds,including dechlorinated griseofulvin (6),griseofulvin (7),dehydrogriseofulvin (8),oxaline (9),4-megastigmen-3,9-dione (10),and p-hydroxyacetophenone (11),were purified and identified from the culture broth of P. coprophilum Mzz9. Compounds 1 and 5 were isolated from B. harlandii for the first time,while compounds 6-11 were identified for the first time from the endophytic fungus of B. harlandii.

Key words: Balanophora harlandii;endophytic fungi, Penicillium coprophilum Mzz9;secondary metabolites;chemical constituents

中图分类号:  R284.1