天然产物研究与开发 ›› 2020, Vol. 32 ›› Issue (9): 1545-1551.doi: 10.16333/j.1001-6880.2020.9.013

• 开发研究 • 上一篇    下一篇

永州薄荷精油成分分析及其与异蛇蛇蜕浸液复配剂抑菌效果研究

王思思#,王斌#,廖阳*,蹇宁彬,于凌一丹,邓冰清,闫荣玲*


  

  1. 1湖南科技学院化学与生物工程学院;2湘南优势植物资源综合利用湖南省重点实验室,永州 425199

  • 出版日期:2020-09-28 发布日期:2020-09-25
  • 基金资助:
    中央引导地方科技发展专项(湘财预[2019]98号,2019XF5065);湖南省教育厅科研项目(17C0676);湖南科技学院青苗支持计划(湘科院校发[2019]47号);大学生创业训练项目(湘科院教发[2019]30号)

Ingredients analysis of Yongzhou mint essential oil and its antibacterial effect mixing with the slough extract of Yongzhou Agkistrodon acutus

WANG Si-si#,WANG Bin#,LIAO Yang*,JIAN Ning-bin,YU Lin-yi-dan,DENG Bing-qin,YAN Rong-ling*#br#

#br#
  

  1. 1College of Life Sciences and Chemistry Engineering,Hunan University of Science and Engineering;2Key Laboratory of Comprehensive Utilization of Dominant Plant Resources in Southern Hunan,Yongzhou 425199,China

  • Online:2020-09-28 Published:2020-09-25

摘要:

为更好开发利用永州异蛇(尖吻蝮蛇)和永州薄荷两种地方特色资源,本研究通过GC-MS法分析了永州薄荷精油的成分,采用药敏纸片法测试了永州薄荷精油、异蛇蛇蜕浸液及二者复配剂的抑菌效果。结果发现,永州薄荷精油中共检出20种成分,其中含量较多的成分为薄荷醇(42.67%)、薄荷酮(27.30%),二者占总含量近70%;另有7种成分占总量比例超过1%,分别是α-蒎烯(3.65%)、β-蒎烯(3.28%)、柠檬烯(5.63%)、正辛醇(1.47%)、异薄荷醇(7.88%)、胡薄荷酮(1.35%)、乙酸薄荷酯(2.70%);还含有2种其他产区薄荷精油中未被检出的萜烯与二氢香芹醇(>0.50%)。永州薄荷精油与异蛇蛇蜕浸提液均对金黄色葡萄球菌、痢疾杆菌表现出抑菌活性,其中薄荷精油对痢疾杆菌的抑制效果较金黄色葡萄球菌更佳,而异蛇蛇蜕浸提液则对二者的抑制活性无显著差异;两种提取物复配液的抑菌效果优于各自单方;相同原液浓度下,二者最佳复配比为薄荷精油:蛇蜕浸提液1∶2(V∶V);复配剂对痢疾杆菌、金黄色葡萄球菌最小抑菌浓度分别为15和10 mg/mL。研究结果为两种地方特色动植物资源的协同开发提供了实验依据。

关键词: 金黄色葡萄球菌, 痢疾杆菌, 抑菌圈, 蛇蜕, 薄荷精油

Abstract:

In order to make better use of the two local characteristic resources of Yongzhou,the composition of the Yongzhou mint essential oil was analyzed by GC-MS,the antimicrobial effect of the mint essential oil and the slough extract of Agkistrodon acutus produced in Yongzhou and their mixture were tested by the method of susceptibility disk test. Results showed that,20 components exist in the essential oil of Yongzhou mint,among which menthol (42.67%) and piperitone (27.30%) were the main components accounting for nearly 70% of the total content,and 7 other components such as α-pinene (3.65%), β-pinene (3.28%),limonene (5.63%),octanol (1.47%),isomenthol (7.88%),pulegone(1.35%) and menthyl acetate (2.70%) were more than 1%;meanwhile,there were two components of terpenes and dihydrocarcinol which were not detected in other producing areas’ mint essential oils were both more than 0.50%;The mint essential oil and the slough extract of Agkistrodon acutus produced in Yongzhou both showed antibacterial activity against Shigella dysenteriens and Staphylococcus aureus;mint essential oil perfomed bettrer against S. dysenteriens than S. aureus,while the slough extract showed no significant difference on antibacterial activity between two species of bacteria;the mixture of mint essential oil and slough extract perform better than both single prepration and the best mixing ratio was 1∶2 (mint∶slough,V∶V) with a same pre-mixing original concentration;the minimum inhibitory concentrations (MIC) against S. dysenteriens and S. aureus of the mixture were 15 and 10 mg/mL,respectively.The results could provide experimental basis for the collaborative development of two local characteristic animal and plant resources.

Key words: Staphylococcus aureus;Shigella dysenteriens, bacteriostatic ring;snake slough mint essential oil

中图分类号:  Q945.1