天然产物研究与开发 ›› 2020, Vol. 32 ›› Issue (增刊1): 53-60.doi: 10.16333/j.1001-6880.2020.S.009

• 研究简报 • 上一篇    下一篇

苗药黔产宽叶缬草根挥发油成分分析及含量测定

王淑亮1,2,4,苑春茂2,4,何莉3,张明2,4,曾艳荣2,4,路贵霖3,李亚男2,4*,郝小江1,2,4*   

  1. 1贵州医科大学 药用植物资源功效利用与开发国家重点实验室;2贵州省中国科学院天然产物化学重点实验室/贵州省天然药物工程研究中心,贵阳 550014;3贵州中医药大学药学院,贵阳 550002;4贵州苗药生物技术有限公司,铜仁 554300


  • 出版日期:2020-06-28 发布日期:2020-07-20
  • 基金资助:
    贵州省中医药管理局中医药、民族医药科学技术项目(QZYY-2019-066);研究生教育创新基地建设项目(GNYL[2017]008号-7-Y);国家基金联合基金(U1812403);贵州省科技计划(黔科合支撑[2018]2824)

Constituents analysis and contents determination of the essential oil from Miao medicine the roots of Valeriana officinalis var. latifolia in Guizhou Province

WANG Shu-liang1,2,4,YUAN Chun-mao2,4,HE Li3,ZHANG Ming2,4,ZENG Yan-rong2,4,LU Gui-lin3,LI Ya-nan2,4*,HAO Xiao-jiang1,2,4*#br#

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  1. 1State Key Laboratory of Functions and Applications of Medicinal Plants,Guizhou Medical University;2Key Laboratory of Natural Product Chemistry of Guizhou Academy of Sciences,Guiyang/Guizhou Provincial Engineering Research Center for Natural Drugs,Guiyang 550014,China;3Guizhou University of Traditional Chinese Medicine,College of Pharmacy,Guiyang 550025,China;
    4 Guizhou Miaoyao Biotechnology Co.,Ltd.,Tongren 554300,China


  • Online:2020-06-28 Published:2020-07-20

摘要:

为苗药宽叶缬草(Valeriana officinalis var. latifolia)根挥发油质量评价提供科学依据和方法,采用GC-MS分析鉴定挥发油中25种化合物并用峰面积归一法计算各成分相对含量。利用GC对挥发油中三个高含量成分(乙酸龙脑酯、莰烯及isobicyclogermacrenal)进行方法学研究,线性范围、稳定性、精密度和加样回收率良好;16批贵州不同产地宽叶缬草挥发油样品中乙酸龙脑酯、莰烯及isobicyclogermacrenal含量分别在34.02%~46.70%、13.09%~18.93%、7.44%~15.18%范围之间。首次从宽叶缬草挥发油中分离并鉴定isobicyclogermacrenal。以三个高含量成分为依据,系统评价黔产宽叶缬草根挥发油含量,为其质量标准的制订打下坚实基础。

关键词: 苗药宽叶缬草根, 挥发油, 成分鉴定, 含量测定

Abstract:

In order to further improve the quality of volatile oil samples from Miao medicine the roots of Valeriana officinalis var. latifolia and provide scientific basis and method for its essential oil evaluation,GC-MS was applied for the analysis of essential oil to identify 25 chemical constituents.The relative contents of each constituent in the oil was determined by area normalization.In addition,the methodological study of 3 major constituents (bornyl acetate,camphene and isobicyclogermacrenal) was conducted with a GC system.Method validation results showed good linearity,stability,precision and a high sample recovery rate.The content of borneol acetate,camphene and isobicyclogermacrenal in 16 batches of volatile oil samples from different regions in Guizhou Province ranged from 34.02% to 46.70%,13.09% to 18.93%,and 7.44% to 15.18%,respectively.This was the first time to isolate and identify isobicyclogermacrenal from the volatile oil in Valeriana officinalis var. latifolia.Three high content components were selected to evaluate the quality of the volatile oil,which could lay a solid foundation for the development of the quality standard of the volatile oil.

Key words: Miao medicine roots of Valeriana officinalis var. latifolia, volatile oil, component identification, contents determination

中图分类号:  R284.1 R917