天然产物研究与开发 ›› 2020, Vol. 32 ›› Issue (增刊1): 147-152.doi: 10.16333/j.1001-6880.2020.S.024

• 开发研究 • 上一篇    下一篇

宽叶独行菜质量标准研究

张玉佩1,2，王卫东1,2，蒋思绒1,2，梅丽娟1，吴楠1,2，杨小兵3，于瑞涛1*   

  1. 1中国科学院藏药研究重点实验室，青海省藏药研究重点实验室，中国科学院西北高原生物研究所，西宁 810008；2中国科学院研究生院，北京 100049；3东莞金美济药业有限公司，东莞 523000

  • 出版日期:2020-06-28 发布日期:2020-07-21
  • 基金资助:
    青海省科技合作项目（2017-HZ-811）；青海省藏药研究重点实验室创新平台发展建设专项（2017-ZJ-Y11）

Study on quality standard of Lepidium latifolium L.

ZHANG Yu-pei1,2,WANG Wei-dong1,2,JIANG Si-rong1,2,MEI Li-juan1,WU Nan1,2,YANG Xiao-bing3,YU Rui-tao1*#br#
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  1. 1Key Laboratory of Tibetan Medicine Research,Qinghai Provincial Key Laboratory of Tibetan Medicine Research,Northwest of Plateau Biology,Chinese Academy of Sciences,Xining 810001,China, 2Graduate School of Chinese Academy of Sciences,Beijing 100049,China;3Dongguan Jinmeiji Pharmaceutical Co.,Ltd.,Dongguan 523000,China



  • Online:2020-06-28 Published:2020-07-21

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摘要：

制定宽叶独行菜质量标准，为宽叶独行菜的进一步开发利用提供技术支持。按照《中国药典》方法对12个不同样点药材中浸出物、灰分、和水分进行测定；采用薄层色谱法对其进行定性鉴别；采用高效液相色谱法测定药材中槲皮素-3-O-β-D-葡萄糖-7-O-α-L-鼠李糖的含量。结合研究结果，暂定宽叶独行菜药材中总灰分不得过12%，醇浸出物不得少于10%，水分不得过7%。标准品在其线性范围内线性关系良好（r≥0.999 4），平均加样回收率98.79%，RSD 2.16%；TLC斑点清晰，分离度良好。槲皮素-3-O-β-D-葡萄糖-7-O-α-L-鼠李糖的精密度、稳定性、重复性试验的RSD值均小于2%。该研究所建标准可用于宽叶独行菜药材的质量控制。

关键词: 宽叶独行菜, 槲皮素-3-O-β-D-葡萄糖-7-O-α-L-鼠李糖, TCL, HPLC

Abstract:

The objective of this study was to provide scientific supports for the utilization and development of Lepidium latifolium L. by setting up its quality control specification.In accordance with the methods of Chinese Pharmacopoeia,the crude drug samples collected from 12 different producing areas were used to determine the contents of extract,ash and moisture.The qualitative identification was determined by thin layer chromatography (TLC) method.The content of quercetin-3-O-β-D-glucopyranoside-7-O-α-L-rhamnopyranosyl was determined by high performance liquid chromatography (HPLC) method.According to the study,the total amount of ash in the samples should not exceed 12%,the alcohol extract should not less than 10%,and the moisture content should not more than 7%.The standard showed good linear relationship within its own range (r ≥0.999 4),whose average recovery was 98.79% with the RSD of 2.16%.The TLC spots were clear with good separation.The precision,stability and repeatability of quercetin-3-O-β-D-glucopyranoside-7-O-α-L-rhamnopyranosyl  were less than 2%.In conclusion,the established method can be used for the quality control of Lepidium latifolium L.

Key words: Lepidium latifolium , L, quercetin-3-O-β-D-glucopyranoside-7-O-α-L-rhamnopyranosyl , , TLC, HPLC

中图分类号:  R931.71

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