天然产物研究与开发 ›› 2021, Vol. 33 ›› Issue (1): 1-7.doi: 10.16333/j.1001-6880.2021.1.001

• 研究论文 •    下一篇

桂皮醛对DSS诱导的溃疡性结肠炎小鼠的保护作用研究

吴柯楠,梁艳妮,张东博,任浪浪,李璐含,于金高,张珍,唐志书*,王征*


  

  1. 陕西中医药大学 陕西中药资源产业化省部共建协同创新中心 省部共建秦药特色资源研究开发国家重点实验室(培育) 陕西省创新药物研究中心,咸阳 712083 

  • 出版日期:2021-01-28 发布日期:2021-01-27
  • 基金资助:
    国家自然科学基金(81803951,81973687);陕西省重点产业创新链项目(2018ZDCXL-SF-01-02-02);陕西省创新人才推进计划-青年科技新星项目(2019KJXX-025)

Protective effects of cinnamaldehyde on DSS induced UC mice

WU Ke-nan,LIANG Yan-ni,ZHANG Dong-bo,REN Lang-lang,LI Lu-han,YU Jin-gao,ZHANG Zhen,TANG Zhi-shu*,WANG Zheng*#br#

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  1. Shaanxi Collaborative Innovation Center of Chinese Medicine Resources Industrialization,State Key Laboratory of Research & Development of Characteristic Qin Medicine Resources(Cultivation),Shaanxi Innovative Drug Research Center,Xianyang 712083,China 

  • Online:2021-01-28 Published:2021-01-27

摘要: 为探讨桂皮醛(CA)对实验性溃疡性结肠炎(UC)小鼠的保护作用及其初步机制,用柳氮磺胺吡啶(300 mg/kg)做为阳性对照,CA分别以150、250、500 mg/kg的剂量对3%葡聚硫酸钠(DSS)诱导的UC小鼠进行灌胃治疗。观察小鼠给药前后状态的变化并进行DAI评分,HE染色法观察小鼠结肠组织形态的变化并进行病理评估;ELISA试剂盒、Western blot技术分别检测小鼠血清中炎症因子及小鼠结肠上皮炎症相关蛋白表达水平的变化。结果显示,与模型组比较,CA在实验剂量范围内可保护UC小鼠结肠组织形态完整,改善结肠黏膜损伤;升高血清中IL-4、IL-10含量(P<0.01),降低IL-6、IL-8及TNF-α含量(P<0.01);上调结肠组织NF-κB在胞质内表达量,并抑制STAT3磷酸化水平。综上所述,CA对UC小鼠具有保护作用,可减轻结肠组织损伤程度,抑制炎症反应,更深入的作用机制还有待进一步研究。


关键词: 桂皮醛, 溃疡性结肠炎, 分子机制, 疾病活动指数

Abstract:

To explore the protective effects and its mechanism of cinnamaldehyde (CA) on ulcerative colitis (UC) mice. Sulfasalazine (300 mg/kg) was used as the positive control,and CA was administered to the UC mice which was induced by 3% dextran sodium sulfate (DSS) at 150, 250, 500 mg/kg doses. Observe the changes in the state of the mice before and after the administration and perform the DAI score,and the morphological changes of mice colon were observed by HE staining and pathological evaluation was performed;ELISA kit and Western blot technology were used to detect the changes of inflammatory factors in the serum of mice and the expression of inflammation-related proteins in the colonic epithelium of mice.The results showed that CA could protect the colonic tissue morphological integrity and improve colonic mucosal injury in UC mice within the experimental dose range;Increase the levels of IL-4, IL-10,reduce the levels of IL-6, IL-8 and TNF-α in serum;up-regulate the expression of NF-κB in the cytoplasm,and inhibit the level of phosphorylated STAT3.In summary,CA has a protective effect on UC mice,which could reduce the degree of colon injury and inhibit inflammation.The more in-depth mechanism need to be further study. 

Key words: cinnamaldehyde, ulcerative colitis, molecular mechanism, DAI

中图分类号:  R961.1