天然产物研究与开发 ›› 2021, Vol. 33 ›› Issue (1): 65-72.doi: 10.16333/j.1001-6880.2021.1.009

• 开发研究 • 上一篇    下一篇

沙棘果渣总黄酮提取工艺优化及抗氧化活性研究

田建华*，张春媛，魏璐   

  1. 山西省林业和草原科学研究院，太原 030002
  • 出版日期:2021-01-28 发布日期:2021-01-28
  • 基金资助:
    国家“十三五”重点研发计划（2016YFD0600801）

Study on the extraction technology and antioxidant activity of total flavonoids from the pomace of sea buckthorn 

TIAN Jian-hua*,ZHANG Chun-yuan,WEI Lu   

  1. Shanxi Academy of Forestry and Grassland Science,Taiyuan 030002,China
  • Online:2021-01-28 Published:2021-01-28

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摘要：

利用果胶酶协同超声波法，对沙棘果渣有效成分总黄酮的提取工艺及其抗氧化活性进行了研究。以提取率为指标，通过酶用量、液料比、乙醇浓度、提取时间、提取温度、超声功率等单因素分析，选定酶用量、液料比、超声提取时间3个因素进行响应面试验，确定提取优化工艺条件为：果胶酶用量5.1%，液料比41∶1，超声提取时间81 min，此条件下，沙棘果渣总黄酮提取率达到8.91 mg/g；以BHT为阳性对照，进行了抗氧化活性研究，得出沙棘果渣总黄酮提取液浓度为0.14 mg/mL时，对DPPH自由基的清除率达到了94.42%；提取液浓度为1.2 mg/mL时，对羟自由基和超氧阴离子的清除率分别为83.10%和43.41%。整体来看，沙棘果渣总黄酮具有较强的抗氧化能力。

关键词: 沙棘果渣, 总黄酮, 果胶酶, 超声波, 响应面法, 抗氧化活性

Abstract:

Using pectinase extraction method assisted with ultrasonic treatment,the extraction technology of total flavonoids from the pomace of sea buckthorn was optimized,and furthermore,the flavonoid antioxidant activity was studied.With extract yield as target,a series of affect factors during the extraction process were studied,including enzyme dose,liquid to solid ratio,ethanol percentage,ultrasonic extraction time,extraction temperature and ultrasonic power.Moreover,the experiments of response surface optimization were carried out with enzyme loading amount,liquid to solid ratio and extraction time as variables.The final optimum extraction condition was projected as 5.1% pectinase loading amount,41∶1 liquid to solid ratio and ultrasonic extraction time 81 min.Under such a condition,the 8.91 mg/g flavonoids yield was obtained.About the flavonoid antioxidant activity,these were resulted that the scavenging rate to DPPH radical was 94.42% at 0.14 mg/mL flavonoid extract concentration of the sea buckthorn pomace;the scavenging rate of hydroxyl radical and superoxide anion were respectively 83.10% and 43.41% at 1.2 mg/mL extract concentration.Generally speaking,total flavonoids from the pomace of sea buckthorn show significant antioxidant capacity.

Key words: sea buckthorn pomace, flavonoids, pectinase, ultrasonic, response surface method, antioxidant activity

中图分类号:  TS255.36

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