天然产物研究与开发 ›› 2021, Vol. 33 ›› Issue (10): 1691-1700.doi: 10.16333/j.1001-6880.2021.10.008

• 研究简报 • 上一篇    下一篇

不同丛枝菌根真菌菌剂对滇重楼及土壤中营养元素的影响

赵顺鑫,魏祖晨,李卓蔚,许凌峰,郭冬琴,周浓,杜慧慧*   

  1. 重庆三峡学院生物与食品工程学院 三峡库区道地药材绿色种植与深加工重庆市工程实验室,重庆 404120
  • 出版日期:2021-10-28 发布日期:2021-10-28
  • 基金资助:
    国家自然科学基金(81260622);重庆市自然科学基金(cstc2018jcyjAX0267);重庆三峡学院人才项目(20RC-12);三峡库区可持续发展研究中心开放基金(2020sxxyjd05)

Effects of different arbuscular mycorrhizal fungicides on Paris polyphylla var. yunnanensis and the nutrient elements in the rhizosphere soil

ZHAO Shun-xin,WEI Zu-chen,LI Zhuo-wei,XU Ling-feng,GUO Dong-qin,ZHOU Nong,DU Hui-hui*   

  1. Laboratory for Green Cultivation and Deep Processing of Three Gorges Reservoir Area’s Medicinal Herbs,College of Life Science and Food Engineering,Chongqing Three Gorges University,Chongqing 404120,China
  • Online:2021-10-28 Published:2021-10-28

摘要:

探究不同丛枝菌根真菌菌剂对滇重楼和土壤不同部位营养元素的影响,为滇重楼生物菌肥的研发提供参考。本文通过盆栽接种不同丛枝菌根真菌菌剂,采用凯氏定氮法、钒钼黄比色法、硫酸-高氯酸消煮法和原子吸收光谱法测定滇重楼和土壤中的氮、磷、钾、钙、镁元素含量。结果表明,不同AM 真菌混剂处理后,滇重楼和土壤不同部位营养成分吸收及积累随生长期的不同而各异,S8处理组可增加滇重楼新、老根茎和叶中氮含量、新根茎中磷的含量(最高6.289 g/kg)以及不同部位钙的含量,其中叶中钙含量最高,为40.323 g/kg,也可增加新根茎中镁的含量,最高可达28.142 g/kg;而不同AM处理组根际土壤中氮、磷、钾、镁的含量变化规律不明显,而根际土壤中钙的含量有不同程度的增加,且S8处理组效果较好,可达125.734 g/kg。相关性分析表明:滇重楼老根茎中氮与钙和镁以及钙和镁之间呈正相关,老根茎中钙含量与根际土壤中钙、镁呈负相关。新根茎中氮与磷、镁与磷和钾呈正相关,而根际土壤中钙与钾呈负相关,与根际土壤中镁呈正相关。综上,不同AM真菌组合能影响滇重楼根茎与其根际土壤大量元素的吸收及蓄积,其中S8处理组(微白巨孢囊霉Gigaspora albida、巨大巨孢囊霉Gigaspora gigantea、美丽盾巨孢囊Scutellospora calospora、亮色盾巨孢囊霉Racocetra fulgida、根内球囊霉Rhizophagus intraradices、近明球囊霉Claroideoglomus claroideum)的效果最佳,可作为滇重楼生物菌肥的优良菌株组合之一。

关键词: 土壤微环境, 滇重楼, 丛枝菌根真菌, 营养元素吸收

Abstract:

In order to explore the effect of different arbuscular mycorrhizal fungal inoculants on different nutrient elements of soil and Paris polyphylla var. yunnanensis,it provides a reference for the development of P. polyphylla var. yunnanensis biological bacterial fertilizer.In this study,the pot inoculation experiment was conducted to inoculate different arbuscular mycorrhizal fungi inoculants during the P. polyphylla var. yunnanensis,the contents of nitrogen (N),phosphorus (P),potassium (K),calcium (Ca) and magnesium (Mg) in P. polyphylla var. yunnanensis and soil were determined by the Kjeldahl method,vanadium-molybdenum yellow colorimetric method,the deboiling method with sulfuric acid and perchloric acid and atomic absorption spectrometry.The results showed that the absorption and accumulation of nutrients elements in different parts of Paris polyphylla var. yunnanensis and soil in different growth stages after treatment with different arbuscular mycorrhizal fungal inoculants.S8 treatment could increase the content of N,P in new rhizomes and leaves (up to 6.289 g/kg) and Ca in different parts of Paris polyphylla var. yunnanensis.The content of Ca in leaves was the highest (40.323 g/kg).The content of Mg in new rhizome was 28.142 g/kg,but the content of N,P,K and Mg in different am treatment groups did not change significantly.However,the content of Ca in soil increased in different degrees,and the effect of S8 treatment group was better,up to 125.734 g/kg.Correlation analysis showed that there was a positive correlation between N and Ca,Mg in old tuber,and between Ca and Mg in the old tuber.Ca in old tuber was negatively correlated with Ca,Mg in soil.N and P,Mg in new rhizome were positively correlated with P and K,while Ca and K in soil were negatively correlated with Mg in soil.In conclusion,different AM fungal inoculants could affect the absorption and accumulation of nutrient elements in Paris polyphylla var. yunnanensis and soil,among which S8 treatment group (G. albida,G.gigantea,S. calospora, R. fulgida,R. intraradices,C. claroideum).It can be used as an excellent strain of biological fertilizer of P. polyphylla var. yunnanensis.

Key words: soil microenvironment, Paris polyphylla var.yunnanensis, arbuscular mycorrhizal fungi, nutrient elements absorption

中图分类号:  R282