天然产物研究与开发 ›› 2021, Vol. 33 ›› Issue (10): 1741-1750.doi: 10.16333/j.1001-6880.2021.10.015

• 开发研究 • 上一篇    下一篇

油菜蜂花粉乙醇提取物对异丙肾上腺素致心肌细胞肥大的保护作用及机制研究

陈立府1，孟菲1,2，李志良1,2，谢仕慧1，史培颖1,3 *   

  1. 1福建农林大学动物科学学院（蜂学学院）；2福建农林大学食品科学学院；3天然生物毒素国家地方联合工程实验室，福州 350002
  • 出版日期:2021-10-28 发布日期:2021-10-28
  • 基金资助:
    现代农业产业技术体系（CARS-45-KXJ20）；福建农林大学科技创新专项（CXZX2020055A）

Study on the protective effect and mechanism of rape bee pollen ethanol extract on cardiomyocyte hypertrophy induced by isoproterenol

CHEN Li-fu1,MENG Fei1,2,LI Zhi-liang1,2,XIE Shi-hui1,SHI Pei-ying1,3 *   

  1. 1 College of Animal Sciences (College of Bee Science),Fujian Agriculture and Forestry University;2 College of Food Science,Fujian Agriculture and Forestry University;3 State and Local Joint Engineering Laboratory of Natural Biotoxins,Fuzhou 350002,China

  • Online:2021-10-28 Published:2021-10-28

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摘要：

为研究油菜蜂花粉乙醇提取物（RBPEE）对异丙肾上腺素（ISO）诱导H9c2心肌细胞肥大的干预作用及机制，本实验采用梯度浓度RBPEE处理心肌细胞24 h后饥饿12 h，再以50 μmol/L ISO损伤48 h，收集上述细胞用于骨架染色、表面积、总蛋白、SOD、GSH和MDA测定以及相关基因表达水平检测。研究显示，RBPEE能够显著降低肥大心肌细胞的表面积、总蛋白含量和心肌细胞肥大标志物ANP、BNP、β-MHC的mRNA表达水平；显著增加SOD活性和GSH含量，降低MDA含量；显著降低炎症相关指标IL-2、IL-6、TNF-α和凋亡相关指标Caspase-3、Caspase-8、Caspase-9、Bax的mRNA表达水平，及显著增加Bcl-2的mRNA表达水平和Bcl-2/Bax比值。结果表明RBPEE对ISO致心肌细胞肥大的保护作用与降低氧化应激水平、抑制炎症反应和抗凋亡作用密切相关。

关键词: 油菜蜂花粉, 异丙肾上腺素, 心肌细胞肥大, 氧化应激, 抗炎, 抗凋亡

Abstract:

In order to investigate the protective effect and mechanism of rape bee pollen ethanol extract (RBPEE) on isoproterenol (ISO)-induced H9c2 cardiomyocyte hypertrophy,in this experiment,the cardiomyocytes were treated with gradient concentration of RBPEE for 24 h,and starved for 12 h,and then injured with 50 μmol/L ISO for 48 h,and the treated cells were collected for cytoskeleton staining,surface area,total protein,SOD,GSH and MDA determination,and related gene expression level detection.The study showed that RBPEE could significantly reduce the surface area,total protein content and the mRNA expression levels of cardiomyocyte hypertrophy markers ANP,BNP and β-MHC of the hypertrophic cardiomyocytes,and significantly increase SOD activity and GSH content,and decrease MDA content,as well as significantly reduce the mRNA expression levels of inflammatory response-related factors IL-2,IL-6,TNF-α and apoptosis-related factors Caspase-3,Caspase-8,Caspase-9 and Bax,and significantly increase Bcl-2 mRNA expression level and Bcl-2/Bax.The results suggested that the protective effect of RBPEE on ISO induced cardiomyocyte hypertrophy was closely related to the reduction of oxidative stress level,the inhibition of inflammatory response and the anti-apoptotic effect.

Key words: rape bee pollen, isoproterenol, cardiomyocyte hypertrophy, oxidative stress, anti-inflammation, anti-apoptosis

中图分类号:  S896.4

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