天然产物研究与开发 ›› 2021, Vol. 33 ›› Issue (11): 1910-1917.doi: 10.16333/j.1001-6880.2021.11.014

• 开发研究 • 上一篇    下一篇

缅栀花提取物的挥发性成分鉴定及其葡萄糖转运机制研究

田迪1,陈锐1,朴永革2*,付祺2,李锋2,李河霖2,李宝志2,桂明英3*   

  1. 1云南农业大学茶学院,昆明 650201;2吉林烟草工业有限责任公司技术中心,长春 130031;3云南省高原特色农业产业研究院,昆明 650201

  • 出版日期:2021-11-28 发布日期:2021-12-02
  • 基金资助:
    云南省重大科技专项-绿色食品国际合作研究中心项目(2019ZG009);吉林烟草技术中心技术服务项目(KX132326);云南省科技项目(YCJ 2020-323)

Study on identification of volatile constituents and glucose transport of the extract of Plumeria

TIAN Di1,CHEN Rui1,PIAO Yong-ge2*,FU Qi2,LI Feng2,LI He-lin2,LI Bao-zhi2,GUI Ming-ying3*   

  1. 1College of Tea,Yunnan Agricultural University,Kunming 650201,China; 2Technical Center of Jilin Tobacco Industry Co.,Ltd.,Changchun 130031,China;3Yunnan Plateau Characteristic Agricultural Industry Research Institute,Kunming 650201,China

  • Online:2021-11-28 Published:2021-12-02

摘要:

缅栀花中含有丰富的功能性化合物。本研究采用荧光葡萄糖类似物2-NBDG葡萄糖摄取试剂盒用荧光酶标仪测定不同浓度的缅栀花提取物(PE)处理下细胞荧光强度,及Western blot法检测C2C12骨骼肌细胞中Akt的磷酸化水平及其他关键因子表达。研究结果表明:与空白组相比,PE作用后的C2C12骨骼肌细胞对葡萄糖摄取量显著提高。C2C12骨骼肌细胞在含有4 mg/mL缅栀花水提取物培养液中培养30 min后,Akt的磷酸化水平有明显提升。同时,缅栀花水提物并没有引起腺苷酸激活蛋白激酶(AMPK)Thr172位点磷酸化水平的上调,说明PE提高C2C12骨骼肌细胞葡萄糖摄取与Akt的磷酸化水平的表达具有密切联系。使用GC-MS对进行成分表征,明确PE成分及含量,通过降糖相关成分分析得出:缅栀花能调控葡萄糖转运可能与缅栀花中某些成分相关,具体是一种或是多种还有待探索。

关键词: 缅栀花, 蛋白激酶B(Akt), C2C12骨骼肌细胞, 机制研究

Abstract:

Plumeria contains rich functional compounds.In this study, 2-NBDG glucose uptake kit was used to measure cell fluorescence intensity under different concentrations of Plumeria  water extract (PE),and the phosphorylation level of Akt and the expression of other key factors in C2C12 skeletal muscle cells were detected by Western blot.The results showed that the glucose intake of C2C12 skeletal muscle cells after PE treatment was significantly increased compared with the blank group.After C2C12 skeletal muscle cells were cultured in the medium containing 4 mg/mL PE for 30 min,the phosphorylation level of Akt was significantly increased.Meanwhile,the extract did not up-regulate the phosphorylation of AMPK Thr172,suggesting that the increase of glucose uptake by PE in C2C12 skeletal muscle cells was closely related to the phosphorylation of Akt.The composition of PE was characterized by GC-MS,and the composition and content of PE were determined.Through the analysis of the related components of hypoglycemic,it was concluded that the regulation of glucose transport in PE might be related to some components in Plumeria ,which one or many are still to be explored.

Key words: Plumeria, Akt, C2C12 skeletal muscle cells, mechanism study

中图分类号:  R917