天然产物研究与开发 ›› 2021, Vol. 33 ›› Issue (12): 2099-2106.doi: 10.16333/j.1001-6880.2021.12.015

• 开发研究 • 上一篇    下一篇

荨麻根质量标志物研究

陈艳,李晓波,贾祎萍,胡海,王梦月*   

  1. 上海交通大学药学院,上海 200240
  • 出版日期:2021-12-28 发布日期:2021-12-30
  • 基金资助:
    上海市卫计委项目(2018Y002);上海市自然科学基金(20ZR1427700);国家自然科学基金(81374067)

Research on the quality marker of Urticae Rhizoma

CHEN Yan,LI Xiao-bo,JIA Yi-ping,HU Hai,WANG Meng-yue*   

  1. School of Pharmacy,Shanghai Jiao Tong University,Shanghai 200240,China
  • Online:2021-12-28 Published:2021-12-30

摘要: 荨麻根为常用中药,但其质量评价缺乏研究,本文首次对其质量标志物进行了研究。首先,考察了荨麻根的主要、特征性成分—荨麻醇苷的生物活性,发现荨麻醇苷具有显著抗炎、抗补体、抗氧化作用。接着,采用UV、HR-MS、NMR、FT-IR、CD等技术,对荨麻醇苷的波谱特征进行了系统研究,补充了UV、IR、CD谱数据,发现不同测试溶剂对其NMR、UV光谱有显著影响。在此基础上,建立了荨麻醇苷的HPLC测定方法,发现不同品种的荨麻根中荨麻醇苷含量悬殊,荨麻醇苷可以作为荨麻根的质量标志物。

关键词: 荨麻根, 质量标志物, 荨麻醇苷, 生物活性, 波谱特征, 含量测定

Abstract:

Urticae Rhizoma is a commonly used traditional Chinese medicine.However,its quality evaluation research has not been reported yet.This research is the first study on the quality marker of Urticae Rhizoma.Firstly,the biological activity of urticoside,the main and characteristic component of Urticae Rhizoma,was investigated.The result indicated that urticoside had the significant anti-inflammatory,anti-complement,and antioxidant activities.Subsequently,a systematic study of the spectral characteristics of urticoside,by using UV,HR-MS,NMR,FT-IR,and CD technologies,was carried out.As results,the UV,IR,and CD spectral data of urticoside were supplemented;the determination solvents had the significant effects on NMR and UV spectra.Finally,the contents of urticoside in Urticae Rhizoma were determined by HPLC method.The determination results revealed that the contents of urticoside in different species of Urticae Rhizoma were very different,and urticoside could be used as the quality marker of Urticae Rhizoma.

Key words: Urticae Rhizoma, quality marker, urticoside, biological activity, spectroscopic characteristics, HPLC determination

中图分类号:  R932