The aim of this study is to investigate the effect of ginseng root extract on RAW264.7 cells proliferation,phagocytosis and autophagy level and their correlation.Cell counting reagent (CCK-8) was used to detect the effect of ginseng root extract in cell proliferation of RAW264.7 cells.Neutral red phagocytosis experiment was used to detect the effect of ginseng root extract in phagocytic activity of RAW264.7 cells.The formation of autophagosome was detected by acridine orange staining (AO staining).Western blot was used to detect the expression changes of autophagy related proteins LC3B and ATG7 and the phosphorylation changes of autophagy related proteins AMPK,AKT,mTOR and ULK1.Autophagy regulator rapamycin and CQ were introduced to research the relationship of autophagy,cell proliferation and phagocytosis induced by ginseng root extract.The Results shown that different concentration of ginseng root extract could promote the proliferation and phagocytosis of RAW264.7 cells,compared with blank control group.Ginseng root extract could increase the number of acidic autophagosomes in Raw264.7 cells.The expressions of LC3B,ATG7 and the protein phosphorylation levels of AMPK and ULK1 were increased in ginseng root extract treated cells.Meanwhile,the phosphorylation levels of AKT and mTOR were decreased.Rapamycin further enhanced the phagocytosis of macrophages,while CQ attenuated the improvement of macrophage phagocytosis induced by ginseng root extract.In conclusion,ginseng root extract may enhance the autophagy level of macrophagy RAW264.7,increase cell proliferation and phagocytosis activity,and has the potential effect of activating macrophages to enhance the immunity of the body.