天然产物研究与开发 ›› 2021, Vol. 33 ›› Issue (3): 402-409.doi: 10.16333/j.1001-6880.2021.3.007

• 研究简报 • 上一篇    下一篇

艾纳香油中抗炎成分的筛选及其对炎性因子的影响

蔡亚玲1,2,廖加美1,2,彭俊超1,2,高月1,2,王万林1,2,易琼2,王鲁2*


  

  1. 1贵州大学药学院;2西南特色药用生物资源开发利用教育部工程研究中心,贵阳 550025
  • 出版日期:2021-03-28 发布日期:2021-04-01
  • 基金资助:
    国家自然科学基金(31760747)

Screening of anti-inflammatory substances from Blumea balsamifera (L.) DC.oil and their effects on inflammatory factors

CAI Ya-ling1,2,LIAO Jia-mei1,2,PENG Jun-chao1,2,GAO Yue1,2,WANG Wan-lin1,2,YI Qiong2,WANG Lu2*   

  1. 1College of Pharmacy,Guizhou University;2Engineering Research Center of the Utilization for Characteristic Bio-Pharmaceutical Resources in Southwest,Ministry of Education,Guiyang 550025,China

  • Online:2021-03-28 Published:2021-04-01

摘要: 为了寻找艾纳香油中的抗炎物质,并研究其对巨噬细胞炎性因子的影响,本文采用动物炎症模型筛选艾纳香油中具有抗炎活性的部分化合物,再检测目标化合物对LPS刺激RAW264.7细胞中相关炎性因子的影响。发现艾纳香油中(-)-芳樟醇、反式-石竹烯抗炎活性最佳,且不同剂量的(-)-芳樟醇、反式-石竹烯均能抑制LTB4、PGE2、NO和iNOS炎症介质的产生,下调TNF-α、IL-1β、COX-2、5-LOX、FLAP、NF-κB-p65的表达。结果表明(-)-芳樟醇、反式-石竹烯是艾纳香油中的重要抗炎物质,它们可通过抑制多种炎症介质和细胞因子以及NF-κB-p65的表达来发挥抗炎作用。


关键词: 艾纳香油, 活性成分, 抗炎机制, RAW264.7细胞

Abstract:

To find the anti-inflammatory substances in Blumea balsamifera oil and study their effects on inflammatory factors of macrophages.In this paper,the anti-inflammatory activities of some compounds in B. balsamifera oil were screened by animal inflammatory model,and then the effects of target compounds on related inflammatory factors in RAW264.7 cells stimulated by LPS were detected.The results indicated that the anti-inflammatory activities of (-)-linalool and trans-caryophyllene were the best in B. balsamifera oil,and different doses of (-)-linalool and trans-caryophyllene could inhibit the production of LTB4,PGE2,NO and iNOS inflammatory mediators and down-regulate the expression of TNF-α,IL-1β,COX-2,5-LOX,FLAP and NF- κB-p65.The results show that (-)-linalool and trans-caryophyllene are important anti-inflammatory substances in B. balsamifera oil,which can exert their anti-inflammatory effects by inhibiting the expression of many kinds of inflammatory mediators,cytokines and NF-κB-p65.

Key words: Blumea balsamifera (L.) DC.oil, active ingredient, anti-inflammatory mechanism, RAW264.7 cell

中图分类号:  R965.1