天然产物研究与开发 ›› 2021, Vol. 33 ›› Issue (5): 727-733.doi: 10.16333/j.1001-6880.2021.5.002

• 研究论文 • 上一篇    下一篇

钩藤UHPLC指纹图谱及化学模式识别研究

黄雯雯1,王晓明1,郭亚卿1,韩立峰1,黄宇虹2,潘桂湘2*   

  1. 1天津中医药大学 天津市中药化学与分析重点实验室,天津 301617;2天津中医药大学第二附属医院,天津 300250
  • 出版日期:2021-05-28 发布日期:2021-06-01
  • 基金资助:
    国家自然科学基金(81303182);国家自然科学基金(81173523);国家重大科技“重大新药创制”专项(2018ZX09734-002)

Study on UHPLC fingerprint and chemical pattern recognition of Uncariae Ramulus Cum Uncis

HUANG Wen-wen1,WANG Xiao-ming1,GUO Ya-qing1,HAN Li-feng1,HUANG Yu-hong2,PAN Gui-xiang2*   

  1. 1 Tianjin University of Traditional Chinese Medicine,Tianjin Key Laboratory of Traditional Chinese Medicine Chemistry and Analysis,Tianjin 301617,China;2Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin 300250,China

  • Online:2021-05-28 Published:2021-06-01

摘要:

利用UHPLC指纹图谱结合化学模式识别技术对钩藤药材进行质量评价。采用ACQUITY UPLC HSS T3色谱柱(2.1 mm × 100 mm,1.8 μm),以乙腈-0.1%甲酸水溶液为流动相梯度洗脱,检测波长254 nm,流速0.3 mL/min,进样量2 μL,柱温30 ℃,采集并记录不同来源的钩藤UHPLC指纹图谱,然后利用相似度评价、聚类分析、主成分分析和正交偏最小二乘判别分析等化学模式识别方法,对不同来源的钩藤药材质量进行分析和评价。所建立的钩藤UHPLC指纹图谱共识别、标定了15个共有峰,经鉴定其中的7个共有峰分别为绿原酸、异帽柱木菲碱、异钩藤碱、钩藤碱、缝籽嗪甲醚、去氢毛钩藤碱、毛钩藤碱;30批不同市售来源钩藤的相似度在0.850~0.995之间。经正交偏最小二乘判别分析筛选出了导致不同批次药材质量差异的5个共有峰。本研究建立的UHPLC指纹图谱结合化学模式识别的方法简单、可靠,可为钩藤的质量评价提供参考。

关键词: 钩藤, 指纹图谱, UHPLC, 化学模式识别

Abstract:

The UHPLC fingerprints and chemical pattern recognition were studied to evaluate the quality of Uncariae Ramulus Cum Uncis(URCU).The chromatographic separation was performed on an ACQUITY UPLC HSS T3 column (2.1 mm × 100 mm,1.8 μm).Acetonitrile-0.1% formic acid solution was used as mobile phase with gradient elution.The detection wavelength was set at 254 nm,the flow rate was 0.3 mL/min,the injection volume was 2 μL,and the column temperature was 30 ℃.The chemical pattern recognition of similarity evaluation,cluster analysis,principal component analysis and orthogonal partial least squares analysis were used to analyze and evaluate the quality of URCU.The established URCU UHPLC fingerprint identified 15 common peaks and 7 common peaks could be identified using standards,including chlorogenic acid, isomitraphylline, isorhynchophylline,rhynchophylline, geissoschizine methylether,hirsuteine and hirsutine.The results about the chemical pattern recognition showed that the similarities of those fingerprints were between 0.850 and 0.995.Furthermore,the quality difference of those URCU was mainly caused by 5 common peaks according to the results of orthogonal partial least squares discriminant analysis.In conclusion,the fingerprints combined with chemical pattern recognition provide a simple and accurate analytical method for the quality evaluation of URCU.

Key words: Uncariae Ramulus Cum Uncis, fingerprint, UHPLC, chemical pattern recognition

中图分类号:  R282.5