天然产物研究与开发 ›› 2021, Vol. 33 ›› Issue (5): 758-766.doi: 10.16333/j.1001-6880.2021.5.006

• 研究简报 • 上一篇    下一篇

UPLC-Q-TOF-MS/MS分析凉粉草多酚组分的化学成分

黄家晋1,黄智霖2,陈鸿庚2,刘坤2,王立抗2,郅慧3,丁利君1,郑俊霞2*   

  1. 1广东工业大学轻工化工学院;2广东工业大学生物医药学院;3广州中医药大学中药学院,广州 510006
  • 出版日期:2021-05-28 发布日期:2021-06-01
  • 基金资助:
    国家自然科学基金面上项目(82073977);国家自然科学基金面上项目(81773593)

Analysis of polyphenols from Mesona chinensis by UPLC-Q-TOF-MS/MS

HUANG Jia-jin1,HUANG Zhi-lin2,CHEN Hong-geng2,LIU Kun2,WANG Li-kang2,ZHI Hui3,DING Li-jun1,ZHENG Jun-xia2*#br#   

  1. 1 School of Chemical Engineering and Light Industry;2School of Biomedical and Pharmaceutical Sciences,Guangdong University of Technology;3School of Pharmaceutical Science,Guangzhou University of Chinese Medicine,Guangzhou 510006,China

  • Online:2021-05-28 Published:2021-06-01

摘要: 岭南特色药材凉粉草(Mesona chinensis Benth.)具有很好的消暑、清热、解毒等功能,被称为“仙草”。本研究采用超高效液相色谱串联四极杆飞行时间质谱(UPLC-Q-TOF-MS/MS),选用Waters Acquity UPLC BEH C18色谱柱(2.1 mm×100 mm,1.7 μm);流动相为0.1%甲酸乙腈溶液(A)-0.1%甲酸水溶液(B),梯度洗脱;电喷雾离子源(ESI),负离子监测。共鉴定出51个化合物,包括40个酚类、3个萜类和8个其它化合物,其中1个苯丙酸、1个苯丙酸二聚体、1个苯丙酸三聚体、3个黄酮和3个萜类首次在凉粉草植物中被发现。

关键词: 凉粉草, 多酚, UPLC-Q-TOF-MS/MS

Abstract:

Meisona chinensis Benth.,a characteristic herb of south of China,is known as “Immortal herb” because of its excellent functions of heat elimination,heat clearing,detoxification and so on.Ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass/mass spectrometry (UPLC-Q-TOF-MS) was selected for the study.A BEH C18 column (2.1 mm × 100 mm,1.7 μm) was used with the mobile phase of eluent (A) MeCN containing 0.1% formic acid and eluent B 0.1% formic acid in water.Q-TOF-MS and electrospray ion (ESI) source were applied for the analysis under the negative ions.Fifty-one compounds were identified including 40 polyphenols,3 terpenoids,and 8 other compounds,whereas 1 phenylpropionic acids,1 lignan dimers,2 lignan trimmers,3 flavonoids and 3 terpenoids were firstly identified in Meisona chinensis Benth.

Key words: Mesona chinensis Benth., polyphenols, UPLC-Q-TOF-MS/MS

中图分类号:  284.1 284.2