天然产物研究与开发 ›› 2021, Vol. 33 ›› Issue (5): 767-772.doi: 10.16333/j.1001-6880.2021.5.007

• 研究简报 • 上一篇    下一篇

九蒸九晒熟地黄中的紫罗兰酮类化合物

张靖柯，吕锦锦，李孟，魏俊俊，郑晓珂*，冯卫生*   

  1. 河南中医药大学 河南省中药开发工程技术研究中心，郑州 450046
  • 出版日期:2021-05-28 发布日期:2021-06-01
  • 基金资助:
    国家重点研发计划中医药现代化研究重点专项（2017 YFC1702800）；河南省高层次人才特殊支持计划“中原千人计划”-中原领军人才（ZYQR201810080）

Ionones from the “nine steaming nine sun-drying” Rehmanniae Radix Praeparata

ZHANG Jing-ke,LYU Jin-jin,LI Meng, WEI Jun-jun,ZHENG Xiao-ke*,FENG Wei-sheng*   

  1. The Engineering and Technology Center for Chinese Medicine Development of Henan Province of Henan University of Chinese Medicine,Zhengzhou 450046,China
  • Online:2021-05-28 Published:2021-06-01

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摘要： 本实验对九蒸九晒熟地黄进行进一步的化学成分研究，采用多种柱色谱技术从“九蒸九晒”熟地黄70%丙酮提取物中分离得到3个紫罗兰酮类化合物，运用现代波谱技术并结合参考文献对其结构进行鉴定，分别为sec-hydroxyaeginetic acid-2-O-β-D-glucopyranoside（1）、frehmaglutin A（2）和dihydroxy-β-ionone（3）。其中化合物1为新化合物，化合物2和3为首次从熟地黄中分离得到。采用MTT法研究化合物1~3对人恶性黑色素瘤A375细胞的细胞毒作用。结果表明，化合物1~3在25 μM浓度下均能显著的降低A375的细胞活力，提示其可能具有A375细胞毒活性。


关键词: 熟地黄, 化学成分, 紫罗兰酮, 细胞毒

Abstract:

Three ionones were isolated from the Rehmanniae Radix Praeparata,which were processed by the traditionally-adopted nine cycles of steaming and sun-drying,by using multiple chromatographic methods and their structures were elucidated as sec-hydroxyaegineticacid-2-O-β-D-glucopyranoside,frehmaglutin A,dihydroxy-β-ionone,from their spectral data and physicochemical properties.Among them,compound 1 is a new ionone glycoside.Compounds 2 and 3 were isolated from the Rehmanniae Radix Praeparata for the first time.The effect of compounds 1-3 on Human malignant melanoma A375 cell was investigated by MTT.The results showed that compounds 1-3 (25 μM) significantly inhibited the cell activities of A375 cells.

Key words: Rehmanniae Radix Praeparata, chemical compositions, ionone, cytotoxicity

中图分类号:  R932

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