天然产物研究与开发 ›› 2021, Vol. 33 ›› Issue (6): 1006-1012.doi: 10.16333/j.1001-6880.2021.6.015

• 开发研究 • 上一篇    下一篇

复方精油对PM2.5诱导小鼠肺微血管内皮细胞内皮间质转化的影响

马柯欣1,2,徐靖斌1,任飞1,许新1,刘超生1,李发胜1*   

  1. 1大连医科大学检验医学院,大连 116044;2沈阳市第四人民医院检验科,沈阳 110031
  • 出版日期:2021-06-28 发布日期:2021-07-02
  • 基金资助:
    国家自然科学基金(5137029);大连医科大学省级大学生创新创业训练计划(201910161093)

Effect of compound essential oils on endothelial-mesenchymal transition of mouse pulmonary vascular endothelial cells induced by PM2.5

MA Ke-xin1,2,XU Jing-bin1,REN Fei1,XU Xin1,LIU Chao-sheng1,LI Fa-sheng1*   

  1. 1College of Medical Laboratory, Dalian Medical University, Dalian 116044, China;2Department of Clincal Laboratory,the Fourth People’s Hospital of Shenyang,Shenyang 110031,China

  • Online:2021-06-28 Published:2021-07-02

摘要: 探讨复方精油对PM2.5诱导小鼠肺微血管内皮细胞(MHC)内皮间质转化(EndMT)的干预作用,并从TGF-β1/SMAD3/p-SMAD3通路分析其可能的调节机制。建立PM2.5诱导小鼠MHC和复方精油干预模型,测定细胞活力和迁移能力的变化;采用RT-qPCR和Western blot检测CDH5、CD31、Col1、Acta2、S100A4和TGF-β信号通路相关因子的水平变化。结果表明复方精油可减弱PM2.5对细胞迁移能力的影响;EndMT相关因子的表达随PM2.5作用时间的延长呈现正向发展趋势,复方精油对PM2.5诱导产生的EndMT有良好的干预作用;TGF-β1、TGF-βR1和p-SMAD3的表达均呈现下降趋势,EndMT相关因子的表达水平也有所逆转。复方精油可缓解PM2.5诱导的EndMT进程,其作用与TGF-β1/SMAD3/p-SMAD3信号通路相关。


关键词: PM2.5, 复方精油, 内皮间质转化(EndMT), TGF-β1/SMAD3/p-SMAD3通路

Abstract:

To investigate the effect of compound essential oils (CEOs) on EndMT of mouse pulmonary vascular endothelial cells (MHC) induced by PM2.5,and discuss the regulatory mechanism of CEOs by the TGF-β1/SMAD3/p-SMAD3 pathway.MHC cells were cultured in vitro.Design the PM2.5 group (500 μg/mL),the CEOs group (PM2.5 500 μg/mL + CEOs 10-5%),the inhibitor of TGF-β pathway SB431542 group (10 μmol/L) and the PM2.5+SB431542 group (500 μg/mL + 10 μmol/L).The changes of cell viability and migration ability were analyzed by cell proliferation experiment and cell scratch experiment.RT-qPCR and Western blot were used to detect the levels of CDH5,CD31,Col1,Acta2,S100A4 and TGF-β signal pathway related factors.The cell viability of MHC began to increase after 36 h in the PM2.5 group (P<0.000 1),and there is no significant difference in the CEOs group.The cell migration increased significantly in thePM2.5 group,and CEOs can attenuate the effect of PM2.5 on cell migration (36 h,P<0.05).The result of RT-qPCR and Western blot showed that the expression of EndMT related factors showed a positive trend with the prolongation of PM2.5.CEOs had a beneficial intervention effect on EndMT induced by PM2.5 exposure.Compared with the PM2.5 group,Western blot showed that the expression of TGF-β1,TGF-βR1 and p-SMAD3 decreased in the CEOs group,and the expression of EndMT related factors was reversed after 48 h.CEOs ameliorates EndMT induced by PM2.5,which is related to TGF-β1/SMAD3/p-SMAD3 pathway.

Key words: PM2.5, compound essential oils, endothelial-mesenchymal transition (EndMT), TGF-β1/SMAD3/p-SMAD3 pathway

中图分类号:  R932