天然产物研究与开发 ›› 2021, Vol. 33 ›› Issue (7): 1081-1085.doi: 10.16333/j.1001-6880.2021.7.001

• 研究论文 •    下一篇

海洋来源硫酸多糖体外抗氧化活性作用的初步研究

杨文盛1,袁春红2,韩华1*   

  1. 1同济大学医学院,上海 200092;2岩手大学农学部食料生产环境学科,岩手 
  • 出版日期:2021-07-28 发布日期:2021-07-29
  • 基金资助:
    国家重点研发计划重点专项(2019YFC0312502)

The antioxidant activity in vitro of sulfated polysaccharides from marine sources

YANG Wen-sheng1,YUAN Chun-hong2,HAN Hua1*   

  1. 1School of Medicine,Tongji University,Shanghai 200092,China;2Department of Food Production and Environmental Management, Faculty of Agriculture,Iwate University,Iwate 020-8550,Japan

  • Online:2021-07-28 Published:2021-07-29

摘要:

利用不同极性的溶剂提取、分离并检测裙带菜、紫菜、羊栖菜、糙海参这四种海洋生物的多糖类抗氧化活性物质,以期筛选出具有显著体外活性的海洋来源抗氧化剂。采用过氧化值(POV)、稳定自由基(DPPH·)、多酚氧化酶(PPO)、超氧阴离子自由基(O2)四种体外筛选方法表征不同来源硫酸多糖产物的抗氧化活性。结果显示,四种硫酸多糖可显著抑制猪油的过氧化,并可清除自由基和超氧阴离子,抑制多酚氧化酶的活性,抗氧化活性呈现出一定的浓度依赖性,显示了不同程度的抗氧化作用。这四种硫酸多糖具有发展为天然高效抗氧化剂的潜力。

关键词: 抗氧化性, POV, DPPH, PPO, 海洋生物, 硫酸多糖

Abstract:

To screen out the marine antioxidants with activity,we extracted and isolated four polysaccharides from Undaria pinnatifida,Porphyra,Sargassum fusiforme and Holothuria scabra through different polar solvents.The antioxidant activities of sulfated polysaccharide were determined via peroxide value (POV),polyphenol oxidase (PPO),and two free radical tests(DPPH·and O2).The results showed that four kinds of sulfated polysaccharides significantly inhibited the peroxidation of lard,scavenged free radicals in DPPH and superoxide anion system,and exhibited considerable antioxidant activities against PPO,with a certain concentration dependence.In conclusion,these four sulfated polysaccharides have the potential to develop into natural antioxidants.

Key words: antioxidant activity, POV, DPPH, PPO, marine organism, sulfated polysaccharide

中图分类号:  R914