天然产物研究与开发 ›› 2021, Vol. 33 ›› Issue (7): 1198-1206.doi: 10.16333/j.1001-6880.2021.7.015

• 开发研究 • 上一篇    下一篇

含氨基酸的LCST型离子液体-水混合体系的相行为研究

李艳1,张莉莉1,聂芳红1,高静1,2*   

  1. 1广东海洋大学食品科技学院,湛江 524088;2广东药科大学食品科学学院,中山 528458
  • 出版日期:2021-07-28 发布日期:2021-07-30
  • 基金资助:
    国家自然科学基金(21706040,22078070);广东省自然科学基金面上项目(2019A1515010640);广东省创新强校特色创新项目(230419064)

Phase behavior of LCST-type ionic liquid-water mixtures containing amino acids

LI Yan1,ZHANG Li-li1,NIE Fang-hong1,GAO Jing1,2*   

  1. 1Collage of Food Science and Technology,Guangdong Ocean University,Zhanjiang 524088,China;2College of Food Science,Guangdong Pharmaceutical University,Zhongshan 528458,China

  • Online:2021-07-28 Published:2021-07-30

摘要:

离子液体四丁基膦三氟乙酸盐([P4444]CF3COO)或三丁基辛基溴化膦([P4448]Br)与水构建的混合体系具有低临界共熔温度(LCST)的相变行为,可以通过控制环境温度实现对目标生物分子的萃取。论文考察氨基酸(amino acid,AA)对[P4444]CF3COO-水和[P4448]Br-水混合体系相转变温度的影响以及氨基酸在两相中的分配特性随温度的变化规律。结果表明,添加质量分数为2%~10%的赖氨酸、丙氨酸、甘氨酸、精氨酸和脯氨酸可增强混合体系的成相能力,随着氨基酸添加量的增加以及温度的升高,离子液体相由下相转移至上相。实验证明氨基酸不仅是萃取目标产物,而且能够协助LCST型离子液体-水混合体系在萃取其他生物分子时调控相平衡,为含离子液体的液-液萃取体系的设计提供了基础数据。

关键词: 离子液体, 水, 混合体系, 低临界共熔温度, 氨基酸, 相行为

Abstract:

The mixtures of ionic liquid (IL) tetrabutylphosphonium trifluoroacetate ([P4444]CF3COO) or tributyloctylphosphonium bromide ([P4448]Br) with water showed a lower critical eutectic temperature (LCST)-type phase behavior.Biomolecules could be extracted with the LCST-type systems by controlling the temperature.The effects of amino acid (AA) on the phase transition temperature of [P4444]CF3COO-water and [P4448]Br-water mixtures were investigated.Moreover,the distribution characteristics of AAs in the two phases were analyzed.Results showed that the addition of lysine,alanine,glycine,arginine and proline with the mass fraction of 2%-10% could enhance the phase formation ability of the mixtures.In addition,the IL-rich phase transferred from the lower phase to the upper phase with the increase amount of AAs and temperature.Herein,AAs were not only the target extractive,but also could assist the LCST-type ionic liquid-water mixtures to regulate phase equilibrium in the extraction of other biomolecules.This study can provide a basic data for the design of selective extraction of biomolecules by liquid-liquid systems containing ILs.

Key words: ionic liquid, water, mixtures, lower critical eutectic temperature, amino acids, phase behavior ,

中图分类号:  TQ201 TQ28