天然产物研究与开发 ›› 2021, Vol. 33 ›› Issue (9): 1513-1518.doi: 10.16333/j.1001-6880.2021.9.009

• 研究简报 • 上一篇    下一篇

牛角瓜茎的化学成分及其细胞毒活性研究

于淼,戴好富,黄圣卓,冉红玲,盖翠娟,蔡彩虹,梅文莉*,王昊*   

  1. 中国热带农业科学院热带生物技术研究所 海南省黎药资源天然产物研究与利用重点实验室;海南热带农业资源研究院,海口 571101
  • 出版日期:2021-09-28 发布日期:2021-09-28
  • 基金资助:
    海南省重点研发计划(ZDYF2019217);中国热带农业科学院基本科研业务费专项(1630052017002);财政部和农业农村部国家现代农业产业技术体系资助(CARS-21)

Study on chemical constituents and their cytotoxic activities from the stems of Calotropis gigantea

YU Miao,DAI Hao-fu,HUANG Sheng-zhuo,RAN Hong-ling,GAI Cui-juan,CAI Cai-hong,MEI Wen-li*,WANG Hao*   

  1. Hainan Key Laboratory of Research and Development of Natural Product from Li Folk Medicine,Institute of Tropical Bioscience 
    and Biotechnology,Chinese Academy of Tropical Agricultural Sciences;Hainan Academy of Tropical Agricultural Resources,Haikou 571101,China

  • Online:2021-09-28 Published:2021-09-28

摘要: 为了研究牛角瓜茎的化学成分及其细胞毒活性,采用硅胶、Sephadex LH-20等多种色谱分离技术从牛角瓜茎乙醇提取物中分离得到10个化合物,通过质谱和核磁共振等波谱技术对其结构进行鉴定,分别为(-)-松脂醇-4′-O-β-D-葡萄糖苷(1)、(+)-pinoresinol-4-O-[6′′-O-vanilloyl]-β-D-glucopyranoside(2)、(-)-jatrointelignan B(3)、(+)-(7R,7′R,7′′S,7′′′S,8S,8′S,8′′S,8′′′S)-4′′,4′′′-dihydroxy-3,3′,3′′,3′′′,5,5′-hexamethoxy-7,9′;7′,9-diepoxy-4,8′′;4′,8′′′-bisoxy-8,8′-dineolignan-7′′,7′′′,9′′,9′′′-tetraol(4)、弗如糖苷(5)、(+)-dehydrovomifoliol(6)、3-吲哚甲酸(7)、2,3-二甲氧基苯甲酸(8)、对羟基苯甲酸(9)、香草酸(10)。其中化合物1346~8为首次从牛角瓜中分离得到。采用MTT法测试化合物1~10对慢性髓原白血病细胞K562、人胃癌细胞SGC-7901、人肺癌细胞A549和宫颈癌细胞HeLa的体外细胞毒活性,结果显示化合物45具有不同程度的细胞毒活性,IC50值范围分别为12.37~49.29和0.29~0.98 μM。

关键词: 牛角瓜, 化学成分, 细胞毒活性

Abstract:

To study chemical constituents and their cytotoxic activity from the stems of Calotropis gigantea,ten compounds were isolated from the ethanol extract of C. gigantea by using various chromatographic techniques including silica gel,Sephadex LH-20,etc.Their structures were elucidated on the basis of spectroscopic data including 1H NMR,13C NMR and MS analysis as (-)-pinoresinol-4-O-β-D-glucoside (1),(+)-pinoresinol 4-O-[6′′-O-vanilloyl]-β-D-glucopyranoside (2),(-)-jatrointelignan B (3),(+)-(7R,7′R,7′′S,7′′′S,8S,8′S,8′′S,8′′′S)-4′′,4′′′-dihydroxy-3,3′,3′′,3′′′,5,5′-hexamethoxy-7,9′;7′,9-diepoxy-4,8′′;4′,8′′′-bisoxy-8,8′-dineolignan-7′′,7′′′,9′′,9′′′-tetraol (4),frugoside (5),(+)-dehydrovomifoliol (6),1H-indole-3-carboxylic acid (7),2,3-dimethoxybenzoic acid (8),p-hydroxybenzoic acid (9),and vanillic acid (10).Compounds 1,3,4 and 6-8 were identified from this plant for the first time.All isolated compounds were evaluated in vitro by MTT assay against chronic myeloid leukemia cell K562,human gastric cancer cell SGC-7901,human lung cancer cell A549 and human cervical cancer cell line HeLa.Compounds 4 and 5 exhibited cytotoxic activity against five human cancer cell lines with IC50 values ranging from 12.37 to 49.29 μM and from 0.29 to 0.98 μM,respectively.

Key words: Calotropis gigantea, chemical constituent, cytotoxic activity

中图分类号:  R284.2