天然产物研究与开发 ›› 2022, Vol. 34 ›› Issue (1): 50-56.doi: 10.16333/j.1001-6880.2022.1.007

• 研究简报 • 上一篇    下一篇

广东紫珠中的萜类化合物及其抗炎活性

黄逸敏,袁欢,陈萍,王涛,彭光天,吴爱芝*   

  1. 广州中医药大学中药学院,广州510006
  • 出版日期:2022-01-28 发布日期:2022-01-28
  • 基金资助:
    国家自然科学基金(82073986)

Terpenoids from Callicarpa kwangtungensis and their anti-inflammatory activity

HUANG Yi-min,YUAN Huan,CHEN Ping,WANG Tao,PENG Guang-tian,WU Ai-zhi*   

  1. School of Pharmaceutical Sciences,Guangzhou University of Chinese Medicine,Guangzhou 510006,China
  • Online:2022-01-28 Published:2022-01-28

摘要:

对岭南药材广东紫珠(Callicarpa kwangtungensis)地上部分进行化学成分研究,得到11个萜类化合物,分别鉴定为sambucunlin A(1)、2α-羟基羽扇豆醇(2)、swinhoeic acid(3)、3β-羟基-乌苏烷-11-烯-13β,28-内酯(4)、蔷薇酸(5)、2α,3β,6β,18β,23-pentahydroxy-olean-12-en-28-oic acid(6)、rel-5-(3S,8S-dihydroxy-1R,5S-dimethyl-7-oxa-6-oxobicyclo-oct-8-yl)-3-methyl-2Z,4E-pentadienoic acid(7)、salvionoside B(8)、齐墩果酸(9)、白桦脂酸(10)和α-香树脂醇(11)。其中,化合物1~46~8为首次从该属植物中分离得到。在化学成分分离基础上,进一步选择脂多糖(LPS)诱导的RAW 264.7小鼠巨噬细胞炎症模型进行萜类化合物抗炎活性测试。结果表明:化合物39具有显著的抗炎活性,对比结构发现,三萜类化合物(3~6911)抗炎活性优于倍半萜类化合物(78)。研究结果广东紫珠萜类成分在抗炎方面的临床应用提供了实验依据。

关键词: 马鞭草科, 广东紫珠, 萜类, 抗炎

Abstract:

Eleven terpenoids were isolated and purified from the ethanol extract of Callicarpa kwangtungensis of Lingnan Herbs.Based on spectral analysis and the comparition of literatures,these compounds were identified as sambucunlin A(1),2α-hydroxy-lupeol(2),swinhoeic acid(3),3β-hydroxy-urs-11-en-13β,28-olide(4),euscaphic acid(5),2α,3β,6β,18β,23-pentahydroxy-olean-12-en-28-oic acid(6),rel-5-(3S,8S-dihydroxy-1R,5S-dimethyl-7-oxa-6-oxobicyclo-oct-8-yl)-3-methyl-2Z,4E-pentadienoic acid(7)and salvionoside B(8),oleanolic acid(9),betulinic acid(10),α-amyrin(11),respectively.Compounds 1-4,6-8 was firstly isolated from the genus of Callicarpa.Further the lipopolysaccharide (LPS)-induced RAW 264.7 mouse macrophages was explored as inflammatory model to measure the anti-inflammatory activity of the terpenoids.The experimental results revealed that compounds 3 and 9 had obvious anti-inflammatory activity.It was found that the anti-inflammatory activity of triterpenoids (3-6,9 and 11)was better than that of sesquiterpenoids (7 and 8).This study provided the experimental basis for the clinical application of C. kwangtungensis in anti-inflammatory fields.

Key words: Verbenaceae,  , Callicarpa kwangtungensis, terpenoids, anti-inflammatory activity

中图分类号:  R284