To study the chemical constituents from the whole plant of Achillea alpine L..Various column chromatography,such as macroporous resin,ODS,Sephadex LH-20 and preparative HPLC were adopted to isolate and purify the compounds,and the structures of compounds 1-16 were identified by NMR,MS and other spectroscopic techniques.Sixteen compounds have been isolated from the 95% ethanol extract of A. alpine,and they were identified as (2E,4E)-N-(2-methylbutyl) deca-2,4-dienamide (1),pellitorine (2),(E,E,Z)-2,4,8-decatrienoicacid isobutylamide-8,9-dehydropellitorine（3）,N-2′-methylbutyl-(E, E)-2,4-decadienam（4）,methyl-(E,E)-2,4,9-oxooctadeca-10,12-dienoate（5）,(S)-14-(E,E)-10,12-methyl 14-hydroxy-9-oxo-octadeca-10,12-dienoate（6）,(E, E)-2,4-undecadiene-8,10-diynamide-N-(2-methylpropyl)（7）,(E, E)-2,4-decadienoic acid p-hydroxyphenethylamide（8）,sinapyl alcohol diisovalerate（9）,(S)-13-hydroxyoctadeca-(Z,E)-9,11-dienoic acid（10）,(E, E)-2,4-decadienamide acid p-methoxyphenethylamide（11）,erythro-N-isobutyl-4,5-dihydroxy-2- (E)-decenamide（12）,3-O-feruloylquinic acid (13),cinnamic acid (14),chlorogenic acid (15),3-O-caffeoyl-5-O-feruloylquinic acid (16).Compound 1 was a new amide.Compounds 4-6,9,10,12,13,16 were isolated from the Achillea genus for the first time.Compounds 8 and 11 were isolated from this plant for the first time.Cytotoxic effects of compounds 1-11 were screened on four different gastric cancer cell lines,and the results showed that compounds 2,5 and 9 had weak activities on MGC-803 cell line at 50 μM with the inhibitory rates at 38.7%,34.7%,31.5%,respectively.