天然产物研究与开发 ›› 2022, Vol. 34 ›› Issue (2): 213-219.doi: 10.16333/j.1001-6880.2022.2.005

• 研究论文 • 上一篇    下一篇

黑果枸杞花青素结构差异对其稳定性及细胞抗氧化活性的影响

邓  楷1,2，欧阳健1,2,3，胡  娜1,3，王洪伦1,3*   

  1. 1中国科学院藏药研究重点实验室，西宁 810008；2中国科学院大学，北京 100049；3湖州中科高原生物创新中心，湖州 313000
  • 出版日期:2022-02-28 发布日期:2022-03-07
  • 基金资助:
    青海省科学技术基金（2020-SF-A2）；浙江省自然科学基金（LGC20B020001）

Effects of structural difference on stability and cellular antioxidative activity of anthocyanins from Lycium ruthenicum Murr.

DENG Kai1,2,OUYANG Jian1,2,3,HU Na1,3,WANG Hong-lun1,3 *   

  1. 1Key Laboratory of Tibetan Medicine Research,Chinese Academy of Science,Xining 810008,China;2University of Chinese Academy of Science,Beijing 100049,China;3Huzhou China-Science Innovation Centre of Plateau Biology,Huzhou 313000,China
  • Online:2022-02-28 Published:2022-03-07

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摘要：

本文研究了黑果枸杞花青素化合物结构对其稳定性和细胞抗氧化活性的影响。通过半制备型HPLC分离纯化得到了4种黑果枸杞花青素化合物并鉴定结构，利用比色法评价了化合物的稳定性、细胞毒实验以及H2O2诱导的SH-SY5Y细胞损伤模型评价了细胞抗氧化活性，并讨论了化学结构对其影响。结果表明反式结构比顺式结构在中性和弱碱性条件下有更好的稳定性，反式结构、糖基的增加以及母核B环上的甲氧基取代均可以通过缩短母核与糖链的分子距离从而提高稳定性，糖基的增加能显著地降低损伤细胞中的乳酸脱氢酶水平，且反式结构较顺式结构更有活性优势。综上所述，黑果枸杞花青素的结构影响稳定性和细胞抗氧化活性，但糖基的增加和酰基的反式异构对二者有更积极的意义。

关键词: 黑果枸杞, 花青素, 化学结构, 稳定性, 细胞抗氧化活性

Abstract:

This study investigates the effect of structural differences on the stability and cellular antioxidative activity of anthocyanins from Lycium ruthenicum Murr..We isolate and identify four kinds of anthocyanin compounds from L. ruthenicum by semi-prepared HPLC.We use the spectrometer tests to evaluate the stability,Cell viability assays and oxidative injured SH-SY5Y cells by H2O2 are used to evaluate the cellular antioxidative activity.Then we discuss the effect of chemical structures on the stability and antioxidative activity.The results demonstrate that the trans structure has better stability than the cis structure under neutral and weakly alkaline conditions;molecular simulation shows that the trans structure,the increase of sugar and the methoxy substitution on the B ring can improve the stability by shortening the molecular distance between core and sugar moiety.Besides,cellular activity experiments indicate that increased sugar moiety can significantly reduce lactate dehydrogenase in damaged cells,and the trans structure has more activity advantages than the cis structure.In conclusion,the structural differences of anthocyanins affect stability and cellular antioxidative activity,whereas the increase of sugar groups and trans structure of acyl groups have a more positive meaning for both.

Key words: Lycium ruthenicum Murr., anthocyanins, chemical structure, stability, cellular antioxidative activity

中图分类号:  TS255.1

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