天然产物研究与开发 ›› 2022, Vol. 34 ›› Issue (2): 255-262.doi: 10.16333/j.1001-6880.2022.2.011

• 开发研究 • 上一篇    下一篇

黄精凝集素PCL-2诱导人前列腺癌LNCap细胞凋亡及机制研究

孙婷婷，刘  洋，李卓柯，宗时宇，支文冰，狄志彪，李  晔，张  红*   

  1. 陕西省中医药研究院，西安 710003
  • 出版日期:2022-02-28 发布日期:2022-03-07
  • 基金资助:
    陕西省重点研发计划（2020SF-217）；陕西省中医药管理局项目（2019-ZZ-JC009）；2019年医疗服务与保障能力提升补助资金（中医药事业传承与发展部分）“全国中药资源普查项目”（财社〔2019〕39号）

Lectin PCL-2 of Polygonatum cyrtonema induces apoptosis of human prostate cancer LNCap cells and its mechanism

SUN Ting-ting,LIU Yang,LI Zhuo-ke,ZONG Shi-yu,ZHI Wen-bing,DI Zhi-biao,LI Ye,ZHANG Hong*   

  1. Shaanxi Academy of Traditional Chinese Medicine,Xi'an 710003,China
  • Online:2022-02-28 Published:2022-03-07

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摘要：

研究黄精凝集素PCL-2对人前列腺癌LNCap细胞生物活性和可能的抗肿瘤机制。从黄精药材中提取分离得到黄精凝集素PCL-2，体外培养LNCap细胞，采用WST-1和集落形成实验评价PCL-2对LNCap细胞的增殖作用；2，7-二氯荧光黄双乙酸盐（DCFH-DA）荧光探针法检测LNCap细胞内ROS含量；qRT-PCR和Western blot法检测PCL-2对LNCap细胞中相关基因和蛋白表达的影响。研究结果显示PCL-2能显著抑制LNCap细胞的增殖，促进细胞中ROS生成；PCL-2通过上调LNCap细胞中Bax、Caspase-3及Caspase-9基因mRNA和蛋白表达并下调Bcl-2基因mRNA和蛋白表达水平诱导LNCap细胞凋亡。本研究结果表明PCL-2诱导LNCap细胞凋亡活性作用显著，可作为抑制前列腺癌的潜在药物。

关键词: 黄精凝集素PCL-2, 前列腺癌LNCap, 细胞凋亡, 机制

Abstract:

In this study,the biological activity of lectin PCL-2 of Polygonatum cyrtonema on human prostate cancer LNCap cells and its possible anti-tumor mechanism were explored.PCL-2 was extracted and isolated from P. cyrtonema fresh medicinal materials,and LNCap cells were cultured in vitro.WST-1 and colony formation experiments were used to detect the proliferation of LNCap cells.The level of ROS in LNCap cells were determined by 2,7-dichlorofluorescein yellow diacetate (DCFH-DA) fluorescence probe method.The expression levels of related genes and proteins in LNCap cells was measured by qRT-PCR and Western blot.Activity study results show that PCL-2 can significantly inhibit the proliferation of LNCap cells,promote the generation of ROS in cells.Mechanism research results show that PCL-2 induces LNCap cells apoptosis by up-regulating the mRNA expression levels of Bax,Caspase-3 and Caspase-9 genes and proteins and down-regulating the mRNA expression levels of Bcl-2 genes and proteins.The results of this study show that PCL-2 has a significant effect in inducing apoptosis of LNCap cells,and it can be used as a potential drug for inhibiting prostate cancer.

Key words: lectin PCL-2 of Polygonatum cyrtonema, prostate cancer LNCap cells, apoptosis, mechanism

中图分类号:  R737.25

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