天然产物研究与开发 ›› 2022, Vol. 34 ›› Issue (3): 407-412.doi: 10.16333/j.1001-6880.2022.3.007

• 研究简报 • 上一篇    下一篇

刺苞老鼠簕的化学成分及其抗炎和抗氧化活性研究

贾慧珍1,2，孙   朋1,2，鲁建美1,2，吴   敏1,2，许又凯1*   

  1. 1中国科学院西双版纳热带植物园 热带植物资源可持续利用重点实验室，勐腊 666303；2中国科学院大学，北京 100049
  • 出版日期:2022-03-28 发布日期:2022-03-29
  • 基金资助:
    中国科学院专项中科院合作局一带一路民族药研究与产业合作（Y6ZK131B01）

Chemical constituents from Acanthus leucostachyus and their anti-inflammatory and antioxidant activities

JIA Hui-zhen1,2,SUN Peng1,2,LU Jian-mei1,2,WU Min1,2,XU You-kai1*   

  1. 1CAS Key Laboratory of Tropical Plant Resources Sciences and Sustainable Use,Xishuangbanna Tropical Botanical Garden,Chinese Academy of Sciences,Mengla 666303,China;2University of Chinese Academy of Sciences,Beijing 100049,China
  • Online:2022-03-28 Published:2022-03-29

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摘要：

利用大孔树脂、硅胶和SephadexLH-20凝胶柱色谱等方法，从刺苞老鼠簕Acanthus leucostachyus Wall.ex Nees全草分离纯化得到13个化合物，通过NMR、MS等波谱数据与文献进行对比分析鉴定化合物结构。这些化合物分别鉴定为2-苯并噁唑啉酮（1）、3-羟基乙酰基吲哚（2）、儿茶酚（3）、香草酸（4）、methyl 3,4-dihydroxy-benzoate（5）、N-(2-hydroxyphenyl)formamide（6）、咔唑（7）、邻苯二甲酰正二丁酯（8）、ethyl p-methoxy-trans-cinnamate（9）、肉桂酸乙酯（10）、豆甾醇（11）、3-hydroxy-4-methoxypyridine（12）、豆甾醇-3-O-β-D-吡喃葡萄糖苷（13）。所有化合物均为首次从该植物中分离得到，其中化合物7为首次从老鼠簕属植物中分离得到。采用DPPH自由基清除法测试分离纯化到的化合物的抗氧化活性，用脂多糖（LPS）诱导小鼠单核巨噬细胞RAW 264.7生成NO炎症模型测试抗炎活性。其中化合物3表现出较好的抗氧化活性，IC50值为105.18 μM；化合物3、6、9表现出较强的抗炎活性，IC50值分别为18.49、22.84、41.22 μM。

关键词: 刺苞老鼠簕, 2-苯并噁唑啉酮, 咔唑生物碱, 抗炎, 抗氧化活性

Abstract:

To study the chemical constituents from the whole herb of Acanthus lecucostachyus.Thirteen compounds (1-13) were isolated and purified by column chromatography on microporous resin,silica gel and SephadexLH-20.The structure of the compounds was identified by comparing their NMR,MS spectroscopic data with those in the literatures.These compounds were identified as 2-benzoxazolinone(1), 3-hydroxylacetyl-indole(2),catechol(3),vanillic acid(4),methyl 3,4-dihydroxy-benzoate(5),N-(2-hydroxyphenyl) formamide(6), carbazole(7),dibutyl phthalate(8),ethyl p-methoxy-trans-cinnamate(9),ethyl cinnamate(10),stigmasterol(11),3-hydroxy-4-methoxypyridine(12),stigmasterol-3-O-β-D-glucopyranoside(13).All compounds were isolated from this plant for the first time,in addition,compound 7 was found in the genus Acanthus for the first time.All isolates were evaluated for antioxidant activity by DPPH free radical scavenging method and anti-inflammatory activities by lipopolysaccharide (LPS) induced mouse monocyte macrophage RAW 264.7 to generate NO inflammatory model.Among those compounds,compound 3 showed moderate antioxidant activity with IC50 value of 105.18 μM;Compounds 3,6 and 9 showed significant anti-inflammatory activity with IC50 values of 18.49,22.84 and 41.22 μM,respectively.

Key words: Acanthus lecucostachyus, 2-benzoxazolinone, carbazole alkaloids, anti-inflammatory, antioxidant activity

中图分类号:  Q501

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