天然产物研究与开发 ›› 2022, Vol. 34 ›› Issue (4): 606-613.doi: 10.16333/j.1001-6880.2022.4.008

• 研究简报 • 上一篇    下一篇

秃杉中taiwanins E和H的分离鉴定及除草、细胞毒性研究

周毅峰1,黄继光2,孔德宁2,唐巧玉1,周利娟2*   

  1. 1湖北民族大学 生物资源保护与利用湖北省重点实验室,恩施 445000;2华南农业大学 天然农药与化学生物学教育部重点实验室;华南农业大学植物保护学院,广州 510642
  • 出版日期:2022-04-29 发布日期:2022-04-29
  • 基金资助:
    生物资源保护与利用湖北省重点实验室2020年开放基金(PT012001);广东省自然科学基金(2018A030313 0093)

Isolation and identification of taiwanins E and H from Taiwania flousiana Gaussen and their  herbicidal activity and cytotoxicity

ZHOU Yi-feng1,HUANG Ji-guang2, KONG De-ning2,TANG Qiao-yu1,ZHOU Li-juan2*   

  1. 1Hubei Key Laboratory of Biological Resources Protection and Utilization,Hubei Minzu University,Enshi 445000,China;2Key Laboratory of Natural Pesticide and Chemical Biology,Ministry of Education,South China Agricultural University;College of Plant Protection,South China Agricultural University,Guangzhou 510642,China
  • Online:2022-04-29 Published:2022-04-29

摘要: 秃杉提取物除草活性筛选结果表明,100 μg/mL的乙酸乙酯层萃取物对胜红蓟的根长和鲜重抑制率均达到100%。通过活性追踪,利用各种色谱和波谱学方法分离鉴定了2种化合物:taiwanin E(1)和taiwanin H(2)。进一步活性测定结果表明,化合物1对龙葵和胜红蓟具有优异的根长抑制活性,处理7天后的IC50值分别为6.84和8.76 μg/mL,化合物2对龙葵、马齿苋、薇甘菊、荷莲荳草的根长IC50值均在10 μg/mL以下;二者对胜红蓟的鲜重抑制活性最高,处理7天后的IC50值分别为24.12和21.31 μg/mL。细胞毒力研究发现taiwanin H能破坏烟草细胞核,并表现出良好的细胞毒性。化合物12的除草活性为首次报道。

关键词: 秃杉, taiwanin E, taiwanin H, 除草活性, 细胞毒性

Abstract:

The herbicidal activity of the extract of Taiwania flousiana Gaussen was evaluated by the cup method.Results showed that the fraction of ethyl acetate at 100 μg/mL completely inhibited the growth of Ageratum conyzoides L.The inhibition rates of root length and fresh weight were 100%.Two active ingredients were isolated and identified by the bioassay-guided isolation.Further,the inhibition rates of root length and fresh weight of the two active ingredients were investigated on 19 weed species.Results showed that taiwanin E showed significant activities on the root length of Solanum nigrum L.and A. conyzoides and the IC50 values of 7 d after the treatment were 6.84 and 8.76 μg/mL,respectively.The IC50 values of 7 d after the treatment of taiwanin H on S. nigrum,Portulaca oleracea L.,Mikania micrantha Kunth and Drymaria diandra Blume were less than 10 μg/mL.As far as the fresh weight was concerned,the two active ingredients showed the best activity on A. conyzoides and the IC50 values of 7 d after the treatment were 24.12 and 21.31 μg/mL,respectively.Generally,the two active ingredients were more active on the broadleaves than the grass weeds and taiwanin H showed a better activity than taiwanin E. Cytotoxicity studies revealed that taiwanin H could destroy the nucleus of the tobacco BY-2 cells and showed cytotoxicity.The herbicidal activities of taiwanins H and E,belonging to lignans,were firstly reported.

Key words: Taiwania flousiana Gausse, taiwanin E, taiwanin H, herbicidal activity, cytotoxicity

中图分类号:  S482.1