天然产物研究与开发 ›› 2022, Vol. 34 ›› Issue (4): 647-655.doi: 10.16333/j.1001-6880.2022.4.013

• 开发研究 • 上一篇    下一篇

濒危蒙药沙冬青毛状根代谢组学分析

王沛雅,方彦昊,郭   琪,张   军,李   鑫,杨   晖*,王治业,杨   涛   

  1. 甘肃省科学院生物研究所 甘肃省微生物资源开发利用重点实验室 “特色微生物与植物资源创新”甘肃省国际科技合作基地,兰州 730000
  • 出版日期:2022-04-29 发布日期:2022-04-29
  • 基金资助:
    甘肃省科技重点研发计划(18YF1WA089);甘肃省科学院应用开发项目(2018JK-04);甘肃省科学院创新团队项目(2019CX004-01)

Metabolomics analysis of hairy roots of endangered Mongolian medicine Ammopiptanthus mongolicus

WANG Pei-ya,FANG Yan-hao,GUO Qi,ZHANG Jun,LI Xing,YANG Hui*,WANG Zhi-ye,YANG Tao   

  1. Key Laboratory of Microbial Resources Exploitation and Application /Gansu International Science and Technology Cooperation Base of Microorganism and Plant Germplasm Resources & Genetic Improvement,Institute of Biology,Gansu Academy of Sciences,Lanzhou 730000,China
  • Online:2022-04-29 Published:2022-04-29

摘要:

为了探讨利用蒙药蒙古沙冬青毛状根生产其药用成分的可能性,本研究采用超高效液相色谱-串联四级杆-飞行时间质谱(UPLC-Q-TOF-MS)技术分别对蒙古沙冬青毛状根和自然根组织进行化学成分分析,并通过主成分分析(PCA)和偏最小二乘判别分析(OPLS-DA)等多元变量分析方法研究二者间的代谢产物差异。结果显示,蒙古沙冬青毛状根和自然根样本在正、负离子模式下分别得到3 982和4 209种代谢物,差异代谢物分别为1 706和1 639种,其中表达上调的代谢物分别有1 455和1 095种;注释到KEGG的差异代谢物有171和134种,主要富集在8大类65条代谢通路:氨基酸代谢、次生代谢产物生物合成、碳水化合物代谢、脂质代谢、辅助因子和维生素代谢、萜类和聚酮化合物代谢、能量代谢及核苷酸代谢等通路;蒙古沙冬青毛状根差异代谢产物主要集中在氨基酸代谢和次生代谢产物生物合成通路中,正离子模式下涉及的差异代谢物分别占37.4%和32.7%,负离子模式下占28.4%和26.1%;次生代谢产物生物合成途径中涉及黄酮/类黄酮生物合成、生物碱生物合成、β-内酰胺菌素生物合成、芥子油苷生物合成、苯丙烷代谢等11条代谢通路,其中黄酮/类黄酮生物合成途径涉及的差异代谢产物最多,占次生代谢产物生物合成途径中差异代谢物的34%,其次是有关生物碱生物合成的差异代谢物,占24%;黄酮/类黄酮生物合成途径中涉及的差异代谢物有88.2%呈现上调。本研究结果可为蒙古沙冬青毛状根代谢产物积累及途径解析提供理论依据。

关键词: 沙冬青, 毛状根, 代谢组学, 次生代谢产物

Abstract:

In order to know the possibility of producing medicinal components from hairy roots of Ammopiptanthus mongolicus,UHPLC-Q-TOF-MS method combined with multivariate statistical analysis was used to analyze the chemical components of hairy roots and roots of A. mongolicus,PCA and OPLS-DA were used for data processing.The results showed that 3 982 and 4 209 metabolites were obtained from the hairy roots and roots of A. mongolicus at positive and negative ion modes,respectively,with 1 706 and 1 639 differential metabolites,among which 1 455 and 1 095 were up-regulated;there were 171 and 134 differential metabolites annotated to KEGG,which were enriched to 65 metabolic pathways,mainly concentrated in amino acid metabolism,secondary metabolites biosynthesis,carbohydrate metabolism,lipid metabolism,cofactors and vitamins metabolism,terpenes and polyketides metabolism,energy metabolism and nucleotide metabolism.The differential metabolites were mainly concentrated in amino acid metabolism and secondary metabolites biosynthesis,37.4% and 32.7% of the differential metabolites at positive ion mode,28.4% and 26.1% of the differential metabolites at negative ion mode.There were 11 pathways involved in the secondary metabolites biosynthesis,including flavone/flavonoid biosynthesis,alkaloid biosynthesis,β-lactam biosynthesis,glucosinolate biosynthesis,phenylpropanoid metabolism,etc.and the flavone/flavonoid biosynthesis pathway involved the most differential metabolites,accounting for 34% of the differential secondary metabolites,followed by the differential metabolites related to alkaloid biosynthesis,accounting for 24%.88.2% of differential metabolites in flavone/flavonoid biosynthesis pathway were up-regulated.The results of this study provide a theoretical basis for the accumulation and pathway elucidation of metabolites in hairy roots of A. mongolicus.

Key words: Ammopiptantus mongolicus, hairy root, metabolomics, secondary metabolite

中图分类号:  R282.6