天然产物研究与开发 ›› 2022, Vol. 34 ›› Issue (4): 665-676.doi: 10.16333/j.1001-6880.2022.4.015

• 开发研究 • 上一篇    下一篇

HPLC指纹图谱结合多模式化学计量学方法评价枳壳药材质量

李   灿1,2,曾   娟1,2,龙雨青1,2,周新茹1,2,杨   敏1,2,童巧珍1,2,周日宝1,2*,刘湘丹1,2*


  

  1. 1湖南中医药大学药学院;2湘产大宗道地药材种质资源及规范化种植重点研究室,长沙 410208
  • 出版日期:2022-04-29 发布日期:2022-04-29
  • 基金资助:
    湖南省科技厅中央引导地方项目(2020ZYQ045);湖南省教育厅重点研究项目(20A383);农业农村部农产品质量安全收贮运管控重点实验室开放课题(S2020KFKT-17);刘良院士工作站指导项目(21YS005)

Evaluation of the quality of Aurantii Fructus by HPLC fingerprint combined with multi-mode chemometrics

LI Can1,2,ZENG Juan1,2,LONG Yu-qing1,2,ZHOU Xin-ru1,2,YANG Min1,2,TONG Qiao-zhen1,2,ZHOU Ri-bao1,2*,LIU Xiang-dan1,2*   

  1. 1School of Pharmacy,Hunan University of Traditional Chinese Medicine;2Key Laboratory of Germplasm Resources and Standardized Planting of Bulk Authentic Medicinal Materials from Hunan,Changsha 410208,China
  • Online:2022-04-29 Published:2022-04-29

摘要:

本研究通过高效液相色谱法(HPLC)指纹图谱对不同产地的枳壳进行质量评价。采用相似度评价、聚类分析、主成分分析和正交偏最小二乘法-判别分析对指纹图谱数据进行统计分析。结果表明11份枳壳样品确定了16个共有峰,其相似度处于0.992~0.999,相似度良好,并对13种化学成分进行了含量测定。聚类分析及主成分分析结果将枳壳聚为4类,主成分综合得分对11份枳壳样品质量进行了评价;正交偏最小二乘法-判别分析结果表明柚皮苷、芸香柚皮苷、橙皮苷、枸橘苷是引起枳壳质量差异的主要成分。本研究建立的枳壳HPLC指纹图谱结合化学计量法的方法简便准确,可为枳壳质量控制和品质评价提供参考依据。

关键词: 枳壳, HPLC指纹图谱, 定量分析, 化学计量分析

Abstract:

In this study,the high performance liquid chromatography (HPLC) fingerprint was used to evaluate the quality of Aurantii Fructus from different habitats.Similarity evaluation,cluster analysis,principal component analysis and orthogonal partial least squares-discriminant analysis were adopted to analyze fingerprint data.The results showed that 11 batches of Aurantii Fructus have 16 common peaks with similarity ranged from 0.992 to 0.999,and the content of 13 chemical components has been determined.According to the cluster analysis and principal component analysis,the samples of Aurantii Fructus could be divided into four types.The comprehensive principal component analysis scores evaluated the quality of different batches of Aurantii Fructus.Orthogonal partial least squares method was used to screen out 4 components with quality differences,including naringin,narirutin,hesperidin and poncirin.In this study,the method combining the HPLC fingerprint of Aurantii Fructus with chemical measurement was simple and accurate,which could provide references for the quality control and evaluation of Aurantii Fructus.

Key words: Aurantii Fructus, HPLC fingerprint, quantitative analysis, chemometric analysis

中图分类号:  R917