天然产物研究与开发 ›› 2022, Vol. 34 ›› Issue (5): 790-799.doi: 10.16333/j.1001-6880.2022.5.007

• 研究简报 • 上一篇    下一篇

基于HS-SPME-GC-MS法测定酸橙不同时期及不同部位的挥发性成分

张睿胤1，成   凤1，郑亚杰1， 陈   鹏2，陆   英1，袁建华3，李先信2*，唐   其1*   

  1. 1湖南农业大学园艺学院，长沙 410128；2湖南省农业科学院 园艺研究所，长沙 410125；3长沙和茂农业开发有限公司，宁乡 410609
  • 出版日期:2022-05-28 发布日期:2022-05-23
  • 基金资助:
    湖南省自然科学基金面上项目（2020JJ4357）；湖南农业大学国家重点实验室培育基地重点项目（19KFXM05）；湖南省大学生创新性实验计划（201910537066）

Determination of volatile components in different periods and parts of Citrus aurantium L. based on HS-SPME-GC-MS

ZHANG Rui-ying1,CHENG Feng1,ZHENG Ya-jie1,CHEN Peng2,LU Ying1,YUAN Jian-hua3,LI Xian-xin2*,TANG Qi1*   

  1. 1College of Horticulture,Hunan Agricultural University,Changsha 410128,China;2Institute of Horticulture,Hunan Academy of Agricultural Sciences,Changsha 410125,China;3Changsha Hemao Agricultural Development Co.,Ltd.,Ningxiang 410609,China
  • Online:2022-05-28 Published:2022-05-23

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摘要：

本文主要是通过不同时期酸橙果实中的挥发性成分测定和定性定量分析，获得其主要挥发性成分的变化趋势。在此基础上再选取关键时期对酸橙不同部位挥发性成分进行检测分析和趋势验证。研究方法主要是采用顶空固相微萃取-气质联用技术（HS-SPME-GC-MS）测定酸橙样品中挥发性成分，通过比对标准品、NIST谱库检索、结合文献对其挥发性成分进行鉴定，并用峰面积归一法测定和比对各组分的相对含量。结果显示，在酸橙果实中共初步鉴定出69种成分，通过对比标准品溶液确定了D-柠檬烯、γ-松油烯、芳樟醇三种成分，其中6月17日与7月1日样品的三者总含量相对较高（38.03%、33.95%）。我们选取7月1日酸橙不同部位样品进行检测分析，共鉴定出64种不同化合物，不同部位之间挥发性成分的组成差异较大。其中根部位共鉴定34种，主要成分为榄香醇（38.24%）、反式-α-香柑油烯（23.85%）等；茎部位共鉴定30种，主要成分为榄香醇（23.98%）、百里酚（17.29%）、反式-α-香柑油烯（13.74%）、芳樟醇（8.92%）等；叶部位鉴定41种，主要成分为芳樟醇（24.51%）、百里酚（23.49%）、榄香醇（10.35%）等；分析不同时期酸橙果实挥发性成分发现，D-柠檬烯等三种化合物的相对含量随着生长期呈现“先逐渐上升后逐渐下降”的趋势；酸橙茎、叶中挥发性成分组成丰富，芳樟醇等单萜类成分相对含量较高。本实验为酸橙果实挥发性成分及非药用部位的综合利用提供了数据和技术参考。

关键词: 酸橙, 不同时期, 不同部位, 挥发性成分, 顶空固相微萃取, 气相色谱-质谱联用法

Abstract:

This research mainly focus on the determination of the volatile components in different periods of Citrus aurantium L. fruit and carry out qualitative and quantitative analysis,which can help the change trend pursue of the main volatile components.The key period was chosen to detect and verfy the volatile components in different parts of C. aurantium.The volatile components in C. aurantium samples were determined by headspace solid phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS).The volatile components were identified by comparing standard samples,NIST database and literature,and the relative contents of each component were determined and compared by peak area normalization method.A total of 69 components were preliminarily identified in C. aurantium fruit,the D-limonene,γ-terpinene and linalool were determined by comparing the standard solution,and the total contents of D-limonene,γ-terpinene and linalool were relatively high in June 17 (8.03%) and July 1 samples (33.95%).Samples from different parts of C. aurantium on July 1 were selected for further analysis,and a total of 64 different compounds were identified,and the composition of volatile components varied greatly among different parts.Among them,34 species were identified in the root part,the main components were elemol (38.24%) and trans-α-mandarin (23.85%),and 30 species in the stem were identified.the main components are elemol (23.98%),thymol (17.29%),trans-α-mandarin (13.74%),linalool (8.92%),etc.41 kinds of leaf parts were identified,and the main components were linalool (24.51%),thymol (23.49%) and elemol (10.35%).The relative content of D-limonene and other three compounds increased in the beginning and then decreased gradually during the growing period.The stems and leaves of C. aurantium are rich in volatile components,and the relative content of monoterpenes such as linalool are relatively high.This experiment provides data and technical reference for the comprehensive utilization of volatile components and non-medicinal parts of C. aurantium.

Key words:

中图分类号:  R284.1

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